Phyllanthus species plants are a rich source of phenolics and widely used due to their medicinal properties. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed using high-pressure liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-QTOF-MS/MS) for the identification and characterization of quercetin, kaempferol, ellagic a...

2. Soxhlation: Soxhlet extraction is only required where the desired compound has a limited solubility in the solvent, and the impurity is insoluble in that solvent. If the desired compound has a high solubility in a solvent then a simple filtration can be used to separate the compound from the insoluble substance [1]. In a conventional soxhlet system plant material is placed in thimble-holder,...

Phyllanthus is a traditional medicinal plant that has been found to have antihepatitis, antibacterial, and anticancer properties. The present studies were to investigate the in vitro molecular mechanisms of anticancer effects of Phyllanthus (P. amarus, P. niruri, P. urinaria, and P. watsonii) plant extracts in human prostate adenocarcinoma. The cancer ten-pathway reporter array was performed an...

In a preliminary study, carriers of hepatitis B virus were treated with a preparation of the plant Phyllanthus amarus for 30 days. 22 of 37 (59%) treated patients had lost hepatitis B surface antigen when tested 15-20 days after the end of the treatment compared with only 1 of 23 (4%) placebo-treated controls. Some subjects have been followed for up to 9 months. In no case has the surface antig...

The response surface methodology using the Box-Behnken design was established to describe supercritical carbon dioxide assisted extraction of phyllanthin from Phyllanthus amarus Schum and Thonn leaves prior to HPLC analysis. The effects of extraction pressure, temperature, modifier concentration and extraction time on the yield of phyllanthin were investigated. By solving the regression equatio...

Liver diseases associated with its function in detoxification of xenobiotics are one of the most common causes of death. Side effects of drugs treating liver diseases have marred their popularity. This work was therefore designed to investigate the ameliorating effect of ethanolic leaves extract of phyllantus amarus on carbon tetrachloride (CCl4) induced hepatotocity in albino rats. Thirty six ...