Biosynthetic routes based on cost-efficient, eco-friendly, and sustainable platforms for compounds such as styrene are urgently needed. The biosynthesis of from L-phenylalanine (L-Phe) via trans-cinnamate has long been established, but toxicity limits yields. This study demonstrates that whole-cell cascade biotransformation employing an E. coli consortium expressing respectively phenylalanine a...

L-Phenylalanine is one of the essential amino acids that cannot be synthesized in mammals in adequate amounts to meet the requirements for protein synthesis. Fungi and plants are able to synthesize phenylalanine via the shikimic acid pathway. L-Phenylalanine, derived from the shikimic acid pathway, is used directly for protein synthesis in plants or metabolized through the phenylpropanoid pathw...

The oscillations in phenylalanine aunnnia-lyase activity from Spirodels pelyrhiza and phenylalanine ammonia-lyase and tyrosine ammonia-lyase activities from Lemnas perpusilam disayed a circadian rhythm under continuous lght. Rhythmiclty in enzymic activity could not be detected in continuous darkness since under this condition phenylalanine ammonialyase activity remains at a fairly constantly l...

Phenylketonuria (PKU) is an autosomal recessive genetic disorder in which mutations in the phenylalanine-4-hydroxylase (PAH) gene result in an inactive enzyme (PAH, EC 1.14.16.1). The effect is an inability to metabolize phenylalanine (Phe), translating into elevated levels of Phe in the bloodstream (hyperphenylalaninemia). If therapy is not implemented at birth, mental retardation can occur. P...

The first enzyme of the shikimate pathway, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (EC 4.1.2.15), is induced by wounding potato or tomato tissue. The increase in enzyme activity is associated with elevated amounts of the enzyme as determined by immunoblots. The specific wound-induced protein synthesis is preceded by an increase in the mRNA encoding this enzyme. The induced mRNA of ...

The sequence of a phenylalanine ammonia-lyase (PAL; EC: 4.3.1.24) of the thermophilic and radiotolerant bacterium Rubrobacter xylanophilus (RxPAL) was identified by screening the genomes of bacteria for members of the phenylalanine ammonia-lyase family. A synthetic gene encoding the RxPAL protein was cloned and overexpressed in Escherichia coli TOP 10 in a soluble form with an N-terminal His6-t...

Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, D-4630 Bochum. Bundesrepublik Deutschland Z. Naturforsch. 44c, 357—360 (1989); received January 9, 1989 Dedicated to Professor Achim Trebst on the occasion o f his 60th birthday Anthocyanin, L-a-Aminooxy-ß-phenylpropionic Acid, Phenylalanine Ammonia-Lyase, Hibiscus mutabilis When opening in the morning flowers of Hibiscus mutabilis L. ...