Protein synthesis in heart muscle has been studied in the isolated rat heart perfused with buffer containing glucose and various amino acid mixtures. The rate of synthesis was evaluated by measuring incorporation of 14C-phenylalanine into whole heart protein and myosin. Protein degradation was estimated by measuring the fall in the specific activity of the free phenylalanine pool and the releas...

Phenylalanine Ammonia-Lyase, L-ar-Aminooxy-/?-phenylpropionic Acid, Aromatic Amino Acids, Shikimic Acid, Fagopyrum esculentum L-a-Aminooxy-/?-phenylpropionic acid (AOPP), a potent competitive inhibitor of phenylalanine ammonia-lyase (PAL), blocked light-induced phenylpropanoid synthesis in excised buckwheat hypocotyls and produced an up to 40-fold increase in the endogenous phenylalanine concen...

Intramolecular interactions of the functional groups, carboxylic acid, amino, and phenyl in L-phenylalanine have been revealed through inner shell chemical shift. The chemical shift and electronic structures are studied using its derivatives, 2-phenethylamine (PEA) and 3-phenylpropionic acid (PPA), through substitutions of the functional groups on the chiral carbon C(alpha), i.e., carboxylic ac...

L-phenylalanine (Phe), an essential amino acid, is necessary for the synthesis of proteins such as catecholamine and melanin, also precursor acid L-tyrosine (Tyr). In this paper, optimized structures Phe, were calculated by using semi-empirical models AM1, PM3 MNDO; Density Functional (DFT) at B3LYP Hartreefock methods (HF) with 6-311++G (d,p) level. The energies geometric parameters Kaempferol...

background: taxol is one of the most important anti-cancer drugs, which is obtained from yew trees (taxus sp.). t+he fist step in side chainassembly of taxol is catalyzed by phenylalanine aminomutase, which converts α-phenylalanine to β-phenylalanine.objectives: in this study, for the fist time, we report on the cloning, preliminary expression and characterization of a full-length gene andcdna ...

amino acid dehydrogenases (l-amino acid: oxidoreductase deaminating ec 1.4.1.x) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either nad+, nadp+ or fad. these enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...

Enzymatic cycling assays for the determination of L-phenylalanine and phenylpyruvate in deproteinized tissue extracts are described. Assay 1 couples glutamine transaminase K with L-phenylalanine dehydrogenase. Assay 2 combines phenylalanine dehydrogenase, L-amino acid oxidase, and catalase. In both assays, tyrosine and some other amino acids (or their alpha-keto acid analogs) can replace phenyl...

Muscle protein synthesis and breakdown and amino acid transport were measured in 7 healthy young (30 ± 2 yr) and 8 healthy elderly (71 ± 2 yr) volunteers in the postabsorptive state and during the oral administration of an amino acid mixture withl-[ ring-2H5]phenylalanine infusion, femoral artery and vein catheterization, and muscle biopsies. Phenylalanine first-pass splanchnic extraction was m...

Amino acid dehydrogenases (L-amino acid: oxidoreductase deaminating EC 1.4.1.X) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either NAD+, NADP+ or FAD. These enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...

An efficient primary-amine-directed, palladium-catalyzed C?H halogenation (X=I, Br, Cl) of phenylalanine derivatives is reported on a range quaternary amino acid (AA) thanks to suitable conditions employing trifluoroacetic as additive. The extension this original native functionality-directed ortho-selective was even demonstrated with the more challenging tertiary AA.