This study evaluates the adaptative response to heat (63 degrees C) and the modifications in membrane fatty acid composition of Salmonella senftenberg after its growth in an acidified medium and after its exposure to combinations of acid and cold stresses. Cells were grown in Brain Heart Infusion (BHI) buffered at pH 7.0 and acidified up to pH 4.5 (fresh cultures) and kept at refrigeration temp...

Structural changes of pyrene-labeled and unlabeled poly(maleic acid/octyl vinyl ether) (PMAOVE) and poly(maleic acid/methyl vinyl ether) (PMAMVE) with changes in pH have been investigated in this study. The changes in the photophysical properties of pyrene are interpreted to investigate uncoiling or swelling of the polymeric chains with pH. The vibrational fine structure of the pyrene fluoresce...

We have examined the effects o f chemical modification with tr imethyloxonium (TMO) and changes in external p H on the proper t ies o f acetylcholine (ACh)-activated channels in BC3H-1 cells, a clonal muscle cell line. TMO reacts covalently and specifically with carboxylic acid moieties in proteins to convert them to neutral methyl esters. In BC3H-1 cells TMO modification reduces the whole-cell...

2-Cys peroxiredoxins belonging to the Prx1 subfamily are Cys-based peroxidases that control the intracellular levels of H2O2 and seem to assume a chaperone function under oxidative stress conditions. The regulation of their peroxidase activity as well as the observed functional switch from peroxidase to chaperone involves changes in their quaternary structure. Multiple factors can modulate the ...

We have examined the effects of chemical modification with trimethyloxonium (TMO) and changes in external pH on the properties of acetylcholine (ACh)-activated channels in BC3H-1 cells, a clonal muscle cell line. TMO reacts covalently and specifically with carboxylic acid moieties in proteins to convert them to neutral methyl esters. In BC3H-1 cells TMO modification reduces the whole-cell respo...

The health benefits of fruit and vegetable consumption are well recognized and a recent cancer prevention report suggested modifications to diet that included reductions in red meat and increases in plant foods. However, populations in the US and UK still do not meet the 5-A-Day target for a number of reasons, including motivational issues. A readily accessible measure or biomarker of dietary c...

SEVERINGHAUS, JOHN W. Blood gas calculator. J. Appl. Physiol. 21(3): I 108-1 I 16. 1966.-A slide rule is described whose scales yield direct solutions of the following blood gas problems: r) the whole blood oxygen dissociation curve of man with variable temperature, pH, and base excess; 2) the effect of anaerobic temperature change on blood Pea , Pcoz , and pH; 3) the true oxygen consumption fr...

Abstract Accuracy in prediction of water quality is a recent research domain gaining popularity smart aquaculture. In aquaculture, the modifications parameters possess nonlinearity, dynamicity, unstableness and complexity due to open environment with its surroundings. Conventional methods have several disadvantages like poor generalization, lower accuracy high time complexity. By considering th...

Introduction The production of protein-based medical agents, like monoclonal antibodies (Mabs), by biotechnological processes requires a comprehensive quality control. The pharmaceutical industry and national health authorities support the complete characterization of therapeutic proteins to increase the quality and safety. During numerous and different production steps like fermentation, purif...

In order to investigate whether covalent histone modifications may be involved in early embryonic reprogramming events, changes in global levels of a series of histone tail modifications were studied during oocyte maturation and pre-implantation mouse development using indirect immunofluorescence and scanning confocal microscopy. Results showed that histone modifications could be classified int...