Introduction: Omp31 is animmunodominant and protective antigen conserved in Brucella species and a good candidate for vaccine design. Material & methods: The present study aimed at in silico design of the truncated Omp31 (TOmp31) using bioinformatic tools and to express the selected form in Escherichia coli (E. coli) Results and conclusion: Various bioinformatically calculated scores for the ...

background: toxoplasma gondii is an obligate intracellular parasite and its sexual and asexual cycles, respectively take place in the intestinal epithelial cell of definitive host and tissue of intermediate hosts. congenital toxoplasmosis is more impor tant when the mother acquired the infection during pregnancy period for the first time. serological tests are the only meth ods for diagnosis of...

introduction: omp31 is animmunodominant and protective antigen conserved in brucella species and a good candidate for vaccine design. material & methods: the present study aimed at in silico design of the truncated omp31 (tomp31) using bioinformatic tools and to express the selected form in escherichia coli (e. coli) results and conclusion: various bioinformatically calculated scores for the mo...

results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...

Anti-TNF inhibitors exert their therapeutic effect by inhibition of the excessive amounts TNF-? within body. Recombinant should be produced in a soluble refolded form to investigate effectiveness and efficiency anti-TNF-? compounds. In this research, designed cassette was subcloned pET28a expression vector expressed E. coli BL21 (DE3). The identity protein confirmed through SDS-PAGE Western blo...

objective: helicobacter pylori is a widely distributed gram negative bacterium that infects the human stomach and duodenum. some antibiotic regimens are subjected to cure the infection but the cost of drugs, poor patient compliance and emerging of antibiotic-resistant strains are limiting the usefulness of these antibiotic therapies. therefore, interest in developing a h. pylori vaccine is grow...

background: improving the performance of hiv diagnosis assays is one of the most important ways to reduce hiv transmission. because of the high mutation rate of hiv, it is critical to use the conserved proteins to develop diagnostic immunoassay methods. because integrase is one of the most conserved proteins of hiv, it may be a good target for this purpose. in this paper cloning, purification a...