Ammonium sulfate fractionated proteins of bitter gourd (Momordica charantia) were precipitated using jack bean extract as a source of concanavalin A. Concanavalin A-bitter gourd peroxidase retained nearly 86% original activity. This complex was entrapped into calcium alginate-pectin gel. Entrapped concanavalin A-peroxidase complex retained 51% original activity. Soluble and immobilized peroxida...

fire blight, caused by the bacterium erwinia amylovora, is one of the most destructive diseases of plants in the rosaceae subfamily pomoideae. the objective of this study was to determine the level of resistance of 30 pear cultivars and describe the biochemical changes induced in some resistant and susceptible cultivars. the level of resistance was determined by the length of the fire blight le...

During penetration and infection of the bean leaf by the rust fungus, at 22 h after artificial inoculation, the following sites of peroxidase activity were observed which might influence the interaction of the host and parasite: (I) The guard cell walls of the stoma are covered with a layer that shows peroxidase activity. This activity might influence the infection peg of the fungus. (2) Within...

To assess nickel-induced toxicity in plants, Zea mays seeds were germinated and cultured on nutrient solution with nickel concentrations of 50-200 μM for a period of two weeks. Observed biological makers included biomass, soluble and total protein contents, and the activities of guaiacol peroxidase (GPX), ascorbate peroxidase (APX), catalase (CAT), and phenylalanine ammonia-lyase (PAL) in the l...

the host thyroid. The tumors themselves showed extremely low incorporation, although thyroidectomy of the host significantly increased the incorporation in the tumors. However, results of incorporation experiments do not distinguish the transitional from the autonomous tumors. Tumor growth also caused iodide peroxidase activity in the host thyroid to decline. The enzyme activity in the transiti...

We determined selenium concentrations and activities of the selenoenzyme, glutathione peroxidase (EC 1.11.1.9), in the plasma and erythrocytes of 38 apparently healthy women. We determined selenium concentrations directly by polarized Zeeman-effect flameless atomic absorption spectroscopy. Within-run precision studies for the assays gave CVs of 5.6% for a mean erythrocyte selenium concentration...

to investigate the effect of droughtstress on chlorophyll content, enzymatic responses and some growth characteristics of acacia victoriae seedlings and the effect of super-absorbent polymers (sap) in reducing drought stress, a split plot experiment based on the completely randomized design was conducted. the treatments included four levels of drought stress (15, 30, 60 and 100% of field capaci...

Utilizing cell preparations that contained greater than 90% eosinophils and sodium azide to inhibit the lysosomal enzyme peroxidase, studies of eosinophil bactericidal activity were undertaken. Eosinophils did not kill bacteria as well as neutrophils, principally because of a reduced phagocytic capacity. In contrast to neutrophils, eosinophils had high resting iodination activity but failed to ...