نتایج جستجو برای: permeable membrane
تعداد نتایج: 397599 فیلتر نتایج به سال:
The most widely accepted theory of the restirng potential of muscle is that the electrical potential difference between the inside and outside of a muscle fibre arises from the concentration gradients of the potassium and chloride ions. If we follow Boyle & Conway (1941), the membrane is assumed to be permeable to K and Cl but to be impermeable or sparingly permeable to other ions. Since K is m...
It is unclear that how subcellular organelles respond to external mechanical stimuli. Here, we investigated the molecular mechanisms by which mechanical force regulates Ca(2+) signaling at endoplasmic reticulum (ER) in human mesenchymal stem cells. Without extracellular Ca(2+), ER Ca(2+) release is the source of intracellular Ca(2+) oscillations induced by laser-tweezer-traction at the plasma m...
This paper presents a novel microcompartmentalized reaction system for cell-free protein synthesis using semi-permeable microcapsules. We used a microfluidic device to encapsulate template DNA and enzymes for cell-free protein synthesis into semi-permeable microcapsules composed of alginate and polyethylene imine (PEI). The template DNA was encapsulated into individual microcapsules at the conc...
The resting membrane of a barnacle muscle fiber is mostly permeable to cations in a solution of pH 7.7 whereas it becomes primarily permeable to anions if the pH is below 4.0. Mechanisms of ion permeation for various monovalent cations and anions were investigated at pH 7.7 and 3.9, respectively. Permeability ratios were obtained from the relationship between the membrane potential and the conc...
Dialysis with a low-density polyethylene semipermeable membrane (SPM) is an efficient means of removal of lipids in the determination of bioaccumulative, persistent, halogenated organic compounds. In this study an SPM was used for preconcentration of polybrominated diphenyl ethers (PBDE) from food samples. The method is useful for determination of PBDE and other halogenated persistent organic p...
Reactive oxygen and nitrogen species (RONS) are involved in many biochemical processes, including nitro-oxidative stress that causes cancer cell death, observed therapies such as photodynamic therapy cold atmospheric plasma. However, their mechanisms of action selectivity still remain elusive due to the complexity biological cells. For example, it is not well known how RONS generated by permeat...
The transport step for p-aminohippurate (PAH) from cell to lumen across the luminal membrane of rabbit proximal tubules has not been adequately defined. To examine this process more closely, we determined the effects of possible transport inhibitors and substitutes for chloride on PAH secretion in isolated perfused S2 segments of rabbit proximal tubules. The addition of 4-acetamido-4'-isothiocy...
The opportunistic bacterium Pasteurella multocida is extremely susceptible to the hydrophobic biocide triclosan by virtue of its markedly permeable outer membrane, while the nosocomial pathogen Pseudomonas aeruginosa is intrinsically resistant to levels far exceeding the triclosan aqueous solubility limit. Widespread incorporation of triclosan in health and personal care products has resulted i...
Plants can sense and respond to mechanical stimuli, like animals. An early mechanism of mechanosensing and response is speculated to be governed by as-yet-unidentified sensory complexes containing a Ca(2+)-permeable, stretch-activated (SA) channel. However, the components or regulators of such complexes are poorly understood at the molecular level in plants. Here, we report the molecular identi...
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