BACKGROUND Starch-degrading amylase enzyme is important in biotechnological applications as food, fermentation, textile, paper and pharmaceutical purposes. The aim of current study to isolate alkaline thermostable α-amylase bacteria and then study the composition of medium and culture conditions to optimize cells growth and a-amylase production. MATERIALS AND METHODS Thermophilic amylase prod...

The pleuropneumonia-like organisms (PPLO) are recognized as a group of rather fastidious microorganisms. Various peptones, infusions, and serum supplements have been examined by many investigators relating to the development of a medium satisfactory for the in vitro cultivation of these organisms (Morton et al., 1951; Edward, 1947; and others). However, few reports of examinations of the agar i...

Some years ago it was found that Bacillus subtilis sporulated more profusely in Bacto-peptone broth when a small quantity of activated charcoal was added to the medium. Further studies revealed that although the charcoal was removed from the medium prior to inoculation, the percentage of cells sporulating during short incubation periods was still appreciably greater than in untreated Bacto-pept...

As one of the most effective biosurfactants reported to date, lipopeptides exhibit attractive surface and biological activities have great potential serve as biocatalysts. Low yield, high cost production, purification hinder large-scale applications lipopeptides. Utilization waste materials low-cost substrates for growth biosurfactant producers has emerged a feasible solution economical product...

The effect of some natural wastes (orange pomace, orange peel, lemon pomace, lemon peel, apple pomace, pear peel, banana peel, melon peel and hazelnut shell) on the production of xylanase from Trichoderma harzianum 1073 D3 has been studied and maximum activity has been observed on melon peel (26.5 U/mg of protein) followed by apple pomace and hazelnut shell. Also, molasses could be used as an a...

month were wholly extractives. At the end of the third month, ?38 per cent, of serum albumin and -0734 per cent, of urea were present, with a trace of albumose and peptone. The third, obtained after six months, on the other hand, contained -7 per cent, of coagulable proteid, only *08 per cent, of this being serum globulin, and '1685 per cent, of the different albumoses, of which deuteroalbumose...

A surface viable counting method is described making use of a nichrome wire loop having an external diameter 5 mm, which is streaked on an overdried plate. The loop holds 1/35 ml (0.02857 ml) peptone water. When tried in parallel with pipettes that delivered 1/50 ml (0.02 ml) drops, it gave reproducible results not at all inferior to those obtained with 50-dropping pipettes.

The blood was collected as early as possible after onset of symptoms. In two patients the blood was collected 3 hours after onset, in four 4 hours, in four 5 hours, in five 7 hours and in five 9 hours. In twenty of the patients therefore the blood was collected within 9 hours of the onset of symptoms, in the remaining six after longer periods. Approximately 5 c.cm. 9 blood was withdrawn under t...