Two isoforms of acidic 1,2-a-D-mannosidases have been isolated from culture filtrate of Penicillium citrinum. The pl values of the two forms, designated 1,2-a-mannosidase la and lb, were 4.6 and 4.7 respectively. Isoenzymes Ia and Ib exhibited the same molecular mass which was determined to be 53 kDa by SDS/ PAGE and 54 kDa by gel-permeation chromatography. Enzymes Ia and lb hydrolysed yeast ma...

Various N,N'-diaryl-1,3-thiazole-2,4-diamines (3a-w) were synthesized by a three steps process. The structures of the synthesized compounds were confirmed by spectral data and elemental analyses. All the synthesized compounds were tested against two bacterial strains and two fungal strains. Bacterial strains included Gram positive Staphylococcus aureus and Gram negativeve Escherichia coli and f...

The study was carried out to enumerate fungal contamination, and toxigenicity of Aspergillus flavus strains on cacao beans during drying storage. As many as 3500 g storage were purchased from smallholder farmers the plantation areas at Karo Regency, Northern Sumatera. percentage contaminated by fungi conducted using direct plating. Fungal populations soil determined dilution followed pour plate...

Penicillium notatum is a well-known indoor aeroallergen and is frequently included in skin test panels for allergic diagnosis. On two-dimensional immunoblotting using patients' sera containing IgE and monoclonal antibody D7B8 specific for Pen c 1 of P. citrinum, two allergens with a molecular mass of 33 kDa but different isoelectric points were identified. A novel cDNA coding for Pen n 13 was c...

In the course of our previous work we isolated alkaloids such as agroclavine-I and epoxy-agroclavine-I from Penicillium citrinum VKM FW-800 as a microorganism isolated from permafrost1,2). This fungus was shown to coproduce two other alkaloid compounds, which were extractable only under acidic conditions. Positive staining by Dragendorff's reagent but negative Ehrlich reaction suggested the occ...

The aim of this study was to screen 15 essential oils of selected plant species, viz. Lavandula angustifolia, Carum carvi, Pinus mungo var. pulmilio, Mentha piperita, Chamomilla recutita L., Pinus sylvestris, Satureia hortensis L., Origanum vulgare L., Pimpinella anisum, Rosmarinus officinalis L., Salvia officinalis L., Abietis albia etheroleum, Chamomilla recutita L. Rausch, Thymus vulgaris L....