نتایج جستجو برای: pei

تعداد نتایج: 2797  

Journal: :American journal of physiology. Heart and circulatory physiology 2008
James P Fisher Colin N Young Paul J Fadel

Whether the activation of metabolically sensitive skeletal muscle afferents (i.e., muscle metaboreflex) influences cardiac baroreflex responsiveness remains incompletely understood. A potential explanation for contrasting findings of previous reports may be related to differences in the magnitude of muscle metaboreflex activation utilized. Therefore, the present study was designed to investigat...

Journal: :Biopolymers 2008
Ge Jiang Kitae Park Jiseok Kim Ki Su Kim Eun Ju Oh Hyungu Kang Su-Eun Han Yu-Kyoung Oh Tae Gwan Park Sei Kwang Hahn

A novel target specific small interfering RNA (siRNA) delivery system was successfully developed using polyethyleneimine (PEI)-hyaluronic acid (HA) conjugate. Anti-PGL3-Luc siRNA was used as a model system suppressing the PGL3-Luc gene expression. The siRNA/PEI-HA complex with an average size of ca. 21 nm appeared to be formed by electrostatic interaction between the negatively charged siRNA an...

Journal: :BMC Biotechnology 2009
Shuai Shi QingFa Guo Bing Kan ShaoZhi Fu XiuHong Wang ChangYang Gong HongXin Deng Feng Luo Xia Zhao YuQuan Wei ZhiYong Qian

BACKGROUND Polyethyleneimine (PEI), a cationic polymer, is one of the successful and widely used vectors for non-viral gene transfection in vitro. However, its in vivo application was greatly limited due to its high cytotoxicity and short duration of gene expression. To improve its biocompatibility and transfection efficiency, PEI has been modified with PEG, folic acid, and chloroquine in order...

2015
Haidong Cai Yu Qiao Ming Sun Xueyu Yuan Qiong Luo Yuehua Yang Shidong Yuan Zhongwei Lv

BACKGROUND To investigate the in vitro inhibitory effects of PEI-RGD/125I-(αv)ASODN (PEI, polyethylenimine; RGD, Arg-Gly-Asp; ASODN, antisense oligodeoxynucleotide) on the growth and invasion of HepG2 cells. MATERIAL AND METHODS ASODN of the integrin αv-subunit was marked with 125I and underwent complexation with PEI-RGD, a PEI derivative. Next, PEI-RGD/125I-(αv) ASODN was introduced into Hep...

Journal: :Journal of controlled release : official journal of the Controlled Release Society 2011
Yanan Yue Fan Jin Rui Deng Jinge Cai Yangchao Chen Marie C M Lin Hsiang-Fu Kung Chi Wu

Our revisit of the complexation between anionic DNA and cationic polyethylenimine (PEI) in both water and phosphate buffered saline (PBS) by using a combination of laser light scattering (LLS) and gel electrophoresis confirms that nearly all the DNA chains are complexed with PEI to form polyplexes when the molar ratio of nitrogen from PEI to phosphate from DNA (N:P) reaches ~3, but the PEI/DNA ...

Journal: Nanomedicine Journal 2017
Mahboobeh Nazari, Mohsen Alipour, Roya Cheraghi Saman Hosseinkhani

Objective(s): PEI based nanoparticle (NP) due to dual capabilities of proton sponge and DNA binding is known as powerful tool for nucleic acid delivery to cells. However, serious cytotoxicity and complicated conditions, which govern NPs properties and its interactions with cells practically, hindered achievement to high transfection efficiency. Here, we have tried to optimize the properties of ...

Journal: :Journal of controlled release : official journal of the Controlled Release Society 2007
Mangesh C Deshpande Mark R Prausnitz

Ultrasound and poly(ethylenimine) (PEI) have each separately been shown to increase DNA transfection efficiency. This study tested the hypothesis that the combination of ultrasound and PEI can have a synergistic effect to increase DNA transfection. This in vitro study assessed transfection efficiency of two different DNA plasmids encoding green fluorescent protein and firefly luciferase in two ...

2004
Stéphanie Grosse Yolande Aron Isabelle Honoré Guiti Thévenot Claire Danel Annie-Claude Roche Michel Monsigny Isabelle Fajac

Background As we have previously shown that lactosylated polyethylenimine (PEI) is the most efficient glycosylated PEI for gene transfer into human airway epithelial cells in primary culture, we have studied here the role of the lactose residue in the enhancement of gene transfer efficiency observed with lactosylated PEI as compared with unsubstituted PEI in immortalized ( CFTE29ocells) and pri...

Journal: :Anticancer research 2012
Lesheng Teng Jing Xie Lirong Teng Robert J Lee

BACKGROUND RNA interference has promising therapeutic potential. However, safe and efficacious delivery systems are necessary for its application in the clinic. MATERIALS AND METHODS An oleic acid (OA) derivative of branched polyethylenimine (PEI, M.W. 2000 Da), PEI-OA, was synthesized and evaluated for small interfering RNA (siRNA) delivery in SK-HEP-1 liver cancer cells stably transfected w...

2011
Thomas Li Ming Wang Tung Kung Wu Yu-Kuo Wang Ming-Te Yang Cheng-Hsiang Chang Jiou Ming Wang Chin-Yuan Chang TienHsiung Thomas Li

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