the pectic enzymes produced in vitro by 8 isolates (5 highly virulent and 3 weakly virulent) of fusarium oxysporum , were detected by spectrophotometry, and characterized by polyacrylamide gel electrophoresis with substrate-containing gels (zymogram). analysis of the polygalacturonase (pg) isozyme banding patterns (zymogram) identified two distinct phenotypes among the isolates from chickpea (c...

It was reported by Beavan & Brown (1949) that cultures of By8aochlamy8 fulva Olliver & Smith produced protopectinase, but neither polygalacturonase nor pectin esterase; they concluded that B. fulva produced a disaggregating enzyme, which lowered the viscosity ofpectin solutions, without the formation of free reducing groups. A re-investigation of the problem has been made, and in a preliminary ...

corn root tip. Iowa State College Jour. Sci. 28: 177-188. 1953. 5. HOGEBOOM, G. H. and SCHNEIDER, W. C. Cytochemical studies. IV. Physical state of certain respiratory enzymes of mitochondria. Jour. Biol. Chem. 194: 513-519. 1952. 6. JAMES, W. 0. The terminal oxidases in the respiration of the embryos and young roots of barley. Proc. Roy. Soc. (London) B 141: 289-299. 1953. 7. KOCH, F. C. and M...

Pectins are polysaccharides ubiquitous in the plant kingdom and constitute the major component of plant cell walls. The pectinases are a group of related enzymes capable of degrading pectin. Therefore, this group of enzymes have been used for decades in the food and winemaking industry for the processing of fruit juices (Mohnen, 2008; Prade et al., 1999; Ribeiro et al., 2010). The pectinases ar...

Pectins are important structural elements in spruce fibres. Alkaline peroxide bleaching of spruce thermomechanical pulp (TMP) causes degradation and demethylation of pectins, yielding high-charge-density pectic acids. The pectic acids in fibres contribute strongly to the negative fibre charge, and the dissolved pectic acids increase the cationic demand of bleached TMP water. In this study, a me...

BACKGROUND Pectic acid extracted from plants increases the secretion of prolactin (PRL) when injected intravenously into ewes or fed to rats. Fragments of ewe hypophysis and lactating rabbit mammary gland incubated in vitro in the presence of pectic acid secreted more PRL and caseins compared to the controls. However, it is not known whether pectic acid directly stimulates PRL secretion in pitu...

The pectic enzymes in Pectolyase were separated by ion exchange chromatography on Q-Sepharose. Three pectin lyases, two polygalacturonases, and a pectinmethylesterase were resolved. The enzymes were further purified on Mono Q and/or Mono S columns to remove traces of cellulase. The enzymes had molecular weights ranging from 25,000 to 36,000 daltons. They were optimally active between pH 4.0 and...

Degradative enzymes have been used to obtain defined fragments of the isolated cell walls of suspension-cultured sycamore cells. These fragments have been purified and structurally characterized. Fragments released from endopolygalacturonase-pretreated cell walls by a purified endoglucanase and the fragments extracted from these walls by urea and alkali provide evidence for a covalent connectio...

This work investigated the structural, biochemical, and molecular characteristics of grape skin cell wall during ripening, related to susceptibility Botrytis cinerea. The comparative study between two main cultivars in Champagne region, Pinot noir Chardonnay, quantified: (1) maturity physical profile skin; (2) morphological characteristics; (3) soluble pectic polysaccharides located walls; (4) ...