Diagnostic immunoglobulin (Ig) polymerase chain reaction (PCR) clonality analyses need to be simple, reproducible, and rapid. Sucrose and cresol red (gel loading buffer reagents) were added to a routine IgH PCR reaction mix to obviate the need for adding gel loading buffer separately after PCR amplification. Not only did this decrease the time spent after PCR analysis but also gave similar or e...

background: rapid diagnosis and differentiation of brucella is of high importance due to the side effects of antibiotics for the treatment of brucellosis. this study aimed to identify and compare pcr-elisa as a more accurate diagnositc test with other common molecular and serological tests. methods: in this experimental and sectional study, during march 2014 to sep 2015, 52 blood samples of sus...

a total of 105 isolates of erysiphe betae from different regions of iran were investigated. for probable polymorphism of its-5.8s regions using pcr-based rflp, the mentioned fragments were amplified via pcr by its1 and its4 primers and subjected to 1.5% agarose gel electrophoresis. pcr products were digested by haeiii, alui, taqi, hindiii, cfoi, ecori, mspi, saci and rsai res and the digestion ...

We reported a new approach of ABO genotyping by a polymerase chain reaction and restriction fragment length polymorphism method. Instead of amplifying the loci containing the positions of nucleotides 258 and 700 of cDNA of the A transferase separately, we successfully amplified these 2 loci together in one reaction mixture using 2 sets of primers. The amplified DNA products were digested at the...

This research was undertaken to compare the accuracy of Polymerase Chain Reaction, simple and nested assays, in detecting the DNA of the malaria parasite Plasmodium falciparum, to that of expert microscopy, the most commonly used method. Microscopy must detect the whole parasite, whereas PCR can detect only the presence of the parasite’s DNA, which is useful when the parasite is in the dormant ...

A total of 121 reference and clinical strains of both slowly and rapidly growing mycobacteria belonging to 54 species were studied for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock protein. Restriction digests were separated by 10% polyacrylamide gel electrophoresis (PAGE). By including a size standard in each sample, the r...

A loop-mediated isothermal amplification (LAMP) assay using six primers targeting a highly conserved region of the B2L gene has been developed to diagnose orf virus. The assay produces a ladder-like pattern of products on an agarose gel that can be specifically digested with BsrGI enzyme. The sensitivity of the LAMP assay, which was determined to be a single copy of the standard plasmid, was 10...

Hepatitis C virus (HCV) populations in vivo consist of heterogeneous mixtures of genetically different but closely related variants defined as a 'quasispecies'. The longitudinal fluctuation of HCV quasispecies populations in chronic hepatitis C has not been elucidated. Serial plasma samples were obtained from four patients with chronic hepatitis C (two patients without any treatment and two pat...