The use of an adequate concentration of Triton X-100 enhanced immunomagnetic separation of Coxiella burnetii from milk. PCR-enzyme-linked immunosorbent assay (PCR-ELISA) could detect coxiellas more sensitively than could conventional PCR. PCR-ELISA is therefore thought to be suitable for the simultaneous assay of a large number of samples. However, the number of cows from which raw milk tested ...

In 2007, a human Q fever epidemic started, mainly in the south eastern part of The Netherlands with a suspected indirect relation to dairy goats, and, to a lesser degree, to dairy sheep. This article describes the Q fever prevalences in Dutch dairy goat and dairy sheep bulk tank milk (BTM) samples, using a real-time (RT) PCR and ELISA. Results of BTM PCR and ELISA were compared with the serolog...

Aim: To assess the suitability, sensitivity and specificity of F gene based RT-PCR for detection of PPRV infection in India. Materials and Methods: A total of 26 blood samples collected from 26 animals suspected for Peste des petits ruminant (PPR) were tested by RTPCR using F1/F2 primers set and then compared with sandwich-ELISA for detection of PPR virus. Results: Out of 26 samples tested RT-P...

toxoplasmosis is an important zoonotic diseases in human and animals. the disease caused by the protozoan toxoplasma gondii. to determine the infection rate of toxoplasmosis in sheep and goats in east azarbaijan province of iran a total of 186 sera, 13 fetal brains, 13 cotyledons and 34 whole blood samples were collected from sheep and goats in tabriz abattoir during the year 2010. serum sam...

background: the risk of infection by transfusion-transmitted viruses has been reduced remarkably. however, a zero-risk blood supply is still desirable. the screening for antibody to hbc (anti-hbc) has been shown as an alternative test for the detection of hbv infection. objective: the main aim of this study was to evaluate hbv infection markers and the potential value of anti-hbc testing of blo...

background: rapid diagnosis and differentiation of brucella is of high importance due to the side effects of antibiotics for the treatment of brucellosis. this study aimed to identify and compare pcr-elisa as a more accurate diagnositc test with other common molecular and serological tests. methods: in this experimental and sectional study, during march 2014 to sep 2015, 52 blood samples of sus...

AIMS To evaluate different hybridisation techniques to detect and type human papillomavirus (HPV) DNAs amplified by consensus primer polymerase chain reaction (PCR) in biopsy and cytological specimens. METHODS A hybrid capture-immunoassay in microtitre wells was performed to detect HPV sequences amplified by PCR and typed by specific oligoprobes. Consensus primers were used to amplify a seque...

BACKGROUND The use of RT-PCR for diagnosis of group A rotaviruses is increasing, but up to 14% of healthy individuals may be positive by RT-PCR. If RT-PCR is not well correlated with disease, rotavirus A may not always be the cause of illness in RT-PCR positive patients with infectious intestinal disease (IID). OBJECTIVES To describe the differences in faecal viral load between ELISA positive...

Objective. Using the data obtained in Perm Region as an example, to identify effectiveness of polymerase chain reaction (PCR) for diagnosis Human Monocytic Ehrlichiosis (HME) at different periods from onset disease, and determine role HME structure infections transmitted by ixodic ticks using PCR enzyme-linked immunosorbent assay (ELISA)
 Materials methods. A thorough clinical epidemiologi...

The study was made to find out the etiological agent involved in piglet diarrhoea during 20072009 in and around Bareilly. Group A rotavirus (GARV) was found as the main agent using various methods applied to intestinal and fecal samples. Out of 199 necropsied intestinal samples, none was positive in agarose gel electrophoresis, 5 (2.51%) were positive in double antibody sandwich ELISA (DAS-ELIS...