The requirement to monitor the presence of genetically modified organisms (GMO) in a variety of marked products has generated an increasing demand for reliable, rapid, and time and cost-effective analytical methods. Here we report an on-site method for rapid detection of cauliflower mosaic virus promoter (CaMV 35S), a common element present in most GMO, using cross-priming amplification (CPA) t...

Suppression subtractive hybridization (SSH) and differential display reverse transcription PCR (DDRT-PCR) were performed in floral organs of Beta vulgaris monosomic addition line M14 (BvM14) (2n=18+1) and a control of Beta vulgaris L. (2n=18). One of the differential genes was designated as BvM14 rat sarcoma-like protein from brain, i.e., BvM14-Rab. The full length of BvM14-Rab was obtained by ...

The efficiencies of different transformation methods of E. coli DH5Qalpha train, induced by several cations like Mg2+, Mn2+ Rb+ and especially Ca2+, with or without polyethylene glycol (PEG) and dimethyl sulfoxide (DMSO) were compared using the two commonly used plasmid vectors pCAMBIA1201 and pBI121. The widely used calcium chloride (CaCl2) method appeared to be the most efficient procedure, w...

The tree legume leucaena (Leucaena leucocephala) is rich in protein content, but it also contains the toxin mimosine. A set of genes involved in mimosine degradation has been identified from Rhizobium TAL1145. TAL1145 degrades mimosine in two steps: the mid genes encode enzymes that break down mimosine to 3-hydroxy-4-pyridone (HP); HP is further degraded by a dioxygenase and a hydrolase, encode...

This experiment was conducted during the period from August 2004 to April 2005 in the Biotechnology & Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh. In this experiment, an efficient and reproducible protocol for the production of transgenic rice plants was developed by inoculating mature embryo of three indica ric...

Although a great deal of progress has been made toward understanding the role of abscisic acid (ABA) in fruit ripening, many components in the ABA signalling pathway remain to be elucidated. Here, a strawberry gene homologous to the Arabidopsis gene ABI1, named FaABI1, was isolated and characterized. The 1641bp cDNA includes an intact open reading frame that encodes a deduced protein of 546 ami...

The effect of growth regulators and culture conditions on the morphogenetic response of cotyledonary leaf discs was studied in popular cucumber variety (Cucumis sativus cv. Sheetal). Organogenesis was induced directly without any intervening callus phase on Murashige and Skoog medium supplemented with different concentrations of benzyladenine and indole propionic acid. Best results (93%) were o...

The aim of the present study was to genetically modify plantlets of the Chinese yali pear to reduce their expression of ripening-associated 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) and therefore increase the shelf-life of the fruit. Primers were designed with selectivity for the conserved regions of published ACO gene sequences, and yali complementary DNA (cDNA) cloning was performed...

Nanotechnology plays a unique and novel role to develop new methods for genetic engineering. Calcium phosphate (CaP) has various significances in biomedical systems; one of them is plasmid DNA (pDNA) delivery for decades as transfection efficiency relative to the non-viral approaches. In this study, we developed a novel gene transfer carriers, nano-calcium-phosphate (CaP) that provides consiste...

Matrix attachment regions (MARs) are thought to participate in the organization and segregation of independent chromosomal loop domains. Although there are several reports on the action of natural MARs in the context of heterologous genes in transgenic plants, in our study we tested a synthetic MAR (sMAR) with the special property of unpairing when under superhelical strain, for its effect on r...