An ultra scale-down primary recovery sequence was established for a platform E. coli Fab production process. It was used to evaluate the process robustness of various bioengineered strains. Centrifugal discharge in the initial dewatering stage was determined to be the major cause of cell breakage. The ability of cells to resist breakage was dependant on a combination of factors including host s...

In this paper, the discrete element method is used to explore why differing amounts of breakage, quantified using Hardin’s relative breakage parameter (Br), are associated with the critical state line (CSL) and the normal compression line (NCL) at similar stress levels. Virtual samples initially containing more than 20,000 spherical particles were isotropically compressed to a range of confinin...

Tailings dam disasters are reported almost every year and triggered by mechanisms, such as overtopping, piping, others. The failures due to inadequate management cannot be neglected well. These dams constructed mostly in a step-by-step construction method that leads continuously changing state of vertical loads during may lead particle breakage changes strength. Therefore, layer tailings today ...

An experimental study of the impact breakage a single tapioca grain using an air jet mill is carried out. High-velocity jets at circumference cylindrical grinding chamber propel tangentially, resulting in numerous collisions with cylinder walls prior to breakage. Videography and image analysis are used obtain trajectory particle sizes fragments. Each experiment repeated 25 times three different...

Particle breakage is one of the issues that significantly affect strength characteristics aggregate. In this paper, pre-crushed limestone aggregate was evaluated through investigating friction angle, stress–strain behaviour, and single-particle strength. The particle phenomenon studied for samples prepared at different conditions, including non-soaked samples, water-soaked acid-soaked samples. ...

Breakage occurs in several particulate systems that are simulated using the discrete element method (DEM), namely crushing and grinding as well inadvertently others include transportation, handling, mixing, separation geotechnical applications. Different approaches may be used to describe particle breakage DEM, but cases which influences significantly flow behaviour of material, imbedding descr...