Nasopharyngeal secretions from adults and children were obtained in Stockholm, Sweden, for routine diagnosis of influenza A virus, influenza B virus, respiratory syncytial (RS) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. The cells in nasopharyngeal secretions were pelleted by centrifugation for preparation of cell de...

After aerosolization of a bovine strain of parainfluenza type 3 virus, the pathogenesis of the virus was followed from the trachea to the bronchioalveolar compartments of the lung of colostrum-free calves and of conventionally reared calves during a 5- to 12-day postexposure interval. By tissue titration, plaque assay, and electron microscopy, it was found that virus infection could be establi...

Several sulfated polysaccharides (dextran sulfate, pentosan polysulfate, fucoidan, and carrageenans) proved to be potent inhibitors for herpes simplex virus, human cytomegalovirus, vesicular stomatitis virus, Sindbis virus, and human immunodeficiency virus. They were moderately inhibitory to vaccinia virus but not inhibitory to adenovirus, coxsackievirus, poliovirus, parainfluenza virus, and re...

Respiratory disorders are major concern for dairy cattle industry. Viruses and bacteria in combinationwith stress play a key role in triggering acute respiratory infections. The most important viral agents arebovine viral diarrhoea virus (BVDV), bovine herpes virus type 1 (BHV-1), bovine respiratory syncytial virus(BRSV), parainfluenza virus type 3 (PIV-3) and bovine adenovirus (BAV). This cros...

Infection of bovine embryonic lung cells with bovine parainfluenza virus type 3 did not induce in vitro, virus-specific, hemadsorption-related adherence of Corynebacterium pyogenes, Haemophilus somnus, Staphylococcus aureus, Streptococcus zooepidemicus, Pasteurella haemolytica, Listeria monocytogenes, Escherichia coli, Pasteurella multocida, Brucella sp., or Salmonella typhimurium.

From September 1984 to February 1985 a total of three hundred and five specimens of nasopharyngeal secretion (NPS) were examined by the indirect immuno-fluorescence technique to determine the frequency of infections caused by the respiratory syncytial virus (RSV), influenza A, influenza B and parainfluenza viruses, 1 and 3. The specimens were collected from infants and children less than 5 year...

Here, we present the complete genome sequences of two human parainfluenza virus type 3 (HPIV-3) isolates collected from hospitalized infants suffering from acute respiratory disease. These are the first complete genome sequences of HPIV-3 originating from Brazil.

The artificial fusion of cells of different type and species, for the formation of heterokaryons and hybrid cells, is now widely used, since Harris and Watkins (1965) demonstrated the practicality of this technique, with Sendai virus as a fusing agent. A variety of viruses show fusing properties (Poste, 1972)) but in most artificial systems Sendai virus (parainfluenza virus type 1) has been use...