The phospholipid composition of human platelets and erythrocytes has been reported by several investigators (1-4) and the metabolism of phospholipids in human blood has been studied in some detail (5-9). Recent investigations of phospholipid metabolism of leukocytes have been reported (6, 10) but we are not aware of similar studies on platelets. In this laboratory, investigation of the biochemi...

It is widely believed that inhibitory synapses are not present or present in only small numbers in the rodent cerebral cortex during the early postnatal period when the cortex is being innervated by thalamocortical fibers. Quantitative electron microscopy was carried out on the posteromedial barrel subfield of mouse somatosensory cortex from postnatal day 4 (P4) when thalamocortical innervation...

The cytoplasmic assembly of vaccinia virus begins with the transformation of a two-membraned cisterna derived from the intermediate compartment between the endoplasmic reticulum and the Golgi complex. This cisterna develops into a viral crescent which eventually forms a spherical immature virus (IV) that matures into the intracellular mature virus (IMV). Using immunoelectron microscopy, we dete...

Cranial sutures are unique to skull bones and consist of multiple connective tissue cell lineages such as mesenchymal cells, fibroblast-like cells, and osteogenic cells, in addition to osteoclasts. Mechanical modulation of intramembranous bone growth in the craniofacial suture is not well understood, especially during postnatal development. This study investigated whether in vivo mechanical for...

Adhesins and adhesin-related accessory proteins of pathogenic mycoplasmas are required for cytadherence and the subsequent development of disease pathology. The classic example has been Mycoplasma pneumoniae, which causes primary atypical pneumonia in humans. Mutants of M. pneumoniae defective in adhesins (P1 and P30) or in adherence-accessory proteins (HMW1 through HMW4) are unable to colonize...

The equivalent pipe network (EPN) model is an effective way to fluid flow in large-scale fractured rock masses with a complex fracture due its straightforwardness and computational efficiency. This study presents the EPN for characterizing through three-dimensional networks using Monte-Carlo method. extracted from original discrete (DFN) used simulate processes. validity of proposed modeling ap...

In 1952, Kornberg and Pricer (1) reported in a preliminary communication that P-choline1 labeled with P32 and Cl* was converted by liver enzymes to a lipide which was not further characterized. The ratio of P32 to Cl4 in the product was closely similar to that of the labeled P-choline, suggesting incorporation of both phosphorus and choline as an intact unit into a phospholipide, presumably lec...

The synthesis of heterologous proteins in Lactococcus lactis is strongly influenced by the promoter selected for the expression. The nisin A promoter is commonly used for induced expression of proteins in L. lactis, whereas few constitutive promoters (P45 and the weaker P32) have been used for protein expression studies. In this study, eight different putative strong constitutive promoters were...

The “P32” Astronomical Observation Template (AOT) provided a means to map large areas of sky (up to 45×45 arcmin) in the far-infrared (FIR) at high redundancy and with sampling close to the Nyquist limit using the ISOPHOT C100 (3×3) and C200 (2×2) detector arrays on board the Infrared Space Observatory (ISO). However, the transient response behaviour of the Ga:Ge detectors, if uncorrected, can ...