19 p22 pracTical prOcedures illustrates procedures. This week: catheter drainage and support p24 iNNOVaTiONs reports on nurse-led initiatives. This week: improving care in dermatology p26 guided learNiNg includes online cpd. This week: the Mental capacity act – ethics p29 deVelOpMeNT shows how nurses are developing practice. This week: specialist support network p32 researcH presents research p...

PURPOSE Loss of retinal capillaries is an inherent component of late stage autosomal dominant retinitis pigmentosa (ADRP). This study examined the hypothetical role of tissue hyperoxia in this vascular attenuation process and tested the potential of ambient hypoxia to reverse it. METHODS Transgenic mice expressing a mutant opsin gene with a 3-bp deletion of isoleucine at codon 255/256 were us...

In Creutzfeldt-Jakob disease three major peptides cosediment with the infectious agent. These distinct peptides are not present in identical fractions from uninfected brain, and bind to polyclonal antibodies raised against "prion protein" purified by protease treatment. Three similar distinct peptides are also found in scrapie-infected brain fractions purified without the use of proteases. To c...

Lettuce infectious yellows virus (LIYV) is the type member of the genus Crinivirus in the family Closteroviridae. Like many other positive-strand RNA viruses, LIYV infections induce a number of cytopathic changes in plant cells, of which the two most characteristic are: Beet yellows virus-type inclusion bodies composed of vesicles derived from cytoplasmic membranes; and conical plasmalemma depo...

The involvement of caspase-3 in cell death after hypoxia-ischemia (HI) was studied during brain maturation. Unilateral HI was produced in rats at postnatal day 7 (P7), 15 (P15), 26 (P26), and 60 (P60) by a combination of left carotid artery ligation and systemic hypoxia (8% O2). Activation of caspase-3 and cell death was examined in situ by high-resolution confocal microscopy with anti-active c...

Symptomatic grapevine samples were collected from vineyards in Zanjan province to detect Grapevine virus A. Total RNA was extracted from symptomatic leaf samples and subjected to cDNA synthesis using random hexamer primers. Then, a DNA fragment around 800 bp including the complete coat protein (CP) gene was amplified from nine out of 57 samples by polymerase chain reaction (PCR) using specific ...

In situ hybridization technique was applied using a tritiated (3H) bovine IFNG cDNA probe to regionally localize the gene on river and swamp buffalo chromosomes. The hybridization signals peaked on the 4q23-->q26 bands in river buffaloes and on the 1p24-->p26 bands in swamp buffaloes. The results are compared to the localization of the same gene in cattle. Possible evolutionary conservation in ...

23 l p23 UPDATE informs you of changes in clinical practice. This week: patient dignity l p26 PrAcTicAl ProcEDUrEs illustrates a clinical procedure. This week: removing sutures l p28 KNowlEDgE provides guided learning. This week: coPD: part one – diagnosis l p30 DEVEloPmENT highlights how nurses are developing their practice. This week: insulin regimens l p32 rEsEArcH presents the latest nursin...