Type II photodynamic therapy (PDT) is an experimental modality for cancer treatment based on the combined action of a photosensitizer, a special wavelength of light and singlet oxygen (O2) generation. The cell killing is caused by the reaction of cellular acceptors with O2. A mathematical model has been previously developed to incorporate the macroscopic kinetic equations for O2 generation, pho...

Isolated rat hepatocytes in culture were incubated with different concentrations of iron-sorbitol (50, 100, 150, and 200 µM) to assess the changes in reactive oxygen species (ROS) and lipid peroxidation leading to apoptotic hepatocyte cell death. The viability of hepatocytes was declined depending on the iron concentration. One hour incubation of the cells with 100 µM iron resulted in decreased...

Isolated rat hepatocytes in culture were incubated with different concentrations of iron-sorbitol (50, 100, 150, and 200 µM) to assess the changes in reactive oxygen species (ROS) and lipid peroxidation leading to apoptotic hepatocyte cell death. The viability of hepatocytes was declined depending on the iron concentration. One hour incubation of the cells with 100 µM iron resulted in decreased...

Although asbestos stimulates oxygen radical generation in alveolar macrophages, the exact mechanism is still not clear. The purpose of this study was to compare the ability of three asbestos fibers (amosite, chrysotile, and crocidolite) to generate oxygen radicals in macrophages and examine the mechanism of this action. All asbestos fibers were able to induce chemiluminescence but chrysotile in...

Mitochondrial reactive oxygen species (ROS) have emerged as an important mechanism of disease and redox signaling in the cardiovascular system. Under basal or pathological conditions, electron leakage for ROS production is primarily mediated by the electron transport chain and the proton motive force consisting of a membrane potential (ΔΨ) and a proton gradient (ΔpH). Several factors controllin...

Atherosclerosis is the key component of most cardiovascular diseases, including stroke and myocardial infarction. An inflamed endothelium recruits inflammatory cells, such as monocytes, via the expression of various mediators and chemokines. This, in addition to the accumulation of cholesterol and smooth muscle cells (SMCs) in the intima, leads to the transformation of monocytes into foam cells...

Introduction: The current interest in human exploration of the Moon and past experiences of Apollo astronauts has rekindled research into the harmful effects of Lunar dust on human health. While the mineralogical composition of lunar regolith has been well documented, other factors, such as partial melting due to space weathering, UV irradiation, and dryness may also contribute to the toxicity ...

Sepsis and septic shock remain as leading cause of death in adult intensive care units. It is widely accepted that sepsis and septic shock are caused predominantly by gram-negative bacteria and their endotoxins. Endotoxin or Lipopolysaccharide (LPS) have important roles as host responses and trigger the inflammatory processes, caused by gram-negative bacterial infection. Production of oxygen ra...