Pancreatic β-cells use Ca(2+)-dependent exocytosis of large dense core vesicles to release insulin. Exocytosis in β-cells has been studied biochemically, biophysically and optically. We have previously developed a biophysical method to monitor release of endogenous intragranular constituents that are co-released with insulin. This technique involves the expression of ionotropic membrane recepto...

Ultra-short high-power lasers can deliver extreme light intensities ($\ge 10^{20}$ W/cm$^2$ and $\leq 30 f$s) drive large amplitude Surface Plasma Wave (SPW) at over-dense plasma surface. The resulting current of energetic electron has great interest for applications, potentially scaling with the laser amplitude, provided laser-plasma transfer to accelerated particles mediated by SPW is still e...

We consider the evaluation of first-order queries over classes databases that are nowhere dense . The notion was introduced by Nešetřil and Ossona de Mendez as a formalization “sparse” graphs generalizes many well-known graphs, such bounded degree, tree-width, or expansion. It has recently been shown Grohe, Kreutzer, Siebertz databases, sentences can be evaluated in pseudo-linear time (pseudo-l...

The distribution and organization of von Willebrand factor (vWF) multimers on platelets after surface activation have not been fully characterized. In the present study, washed human platelets were allowed to interact with Formvar-coated, electron microscope grids for 20 minutes at 37 degrees C and then fixed. After fixation, cells were washed and then incubated with buffer alone, human plasma,...