Vesicle adhesion and fusion to interfaces are frequently used for the construction of biomimetic surfaces in biosensors and drug delivery. Ubiquitous in cell biology, vesicle fusion involves the transformation of two separate membranes into one contiguous lipid bilayer. In distinction, the deposition of vesicle membranes to hydrophobic surfaces requires the transformation of a lipidic bilayer i...

Reductively [3H]methylated 3H mitochondrial-outer-membrane vesicles from rat liver and vesicles where monoamine oxidase has been derivatized irreversibly by [3H]-pargyline have been deliberately miscompartmentalized by heterologous transplantation into hepatoma (HTC) cells by poly(ethylene glycol)-mediated vesicle-cell fusion. Fluorescein-conjugated mitochondrial-outer-membrane vesicles have al...

The gastric flippase has been found recently in isolated hog gastric vesicles. We found here that the flippase ATP-dependently translocates endogenous phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine from the outer (cytosolic) to inner (luminal) leaflet of the vesicle membrane bilayer or to the vesicle interior. In the experiments, vesicles were incubated in the presence and...

The outer segment is a specialized compartment of vertebrate rod and cone photoreceptor cells where phototransduction takes place. In rod cells it consists of an organized stack of disks enclosed by a separate plasma membrane. Although most proteins involved in phototransduction have been identified and characterized, little is known about the proteins that are responsible for outer segment str...

different strains of escherichia coli (e. coli) from human, chickens, and the common strain between human and chickens were isolated and typed with mono-specific antibody. the e. coli strains from each group of human, chicken and common between human and chicken were selected. the polypeptide patterns of selected strains were analyzed and compared with each other by sodium dodecyle sulphate pol...

A method for separating the outer and inner membranes of Pseudomonas aeruginosa PAO1 in the absence of added ethylenediaminetetraacetic acid was devised. The method yields two outer membrane fractions which show the same protein pattern on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, but differ substantially in their relative contents of phospholipids. One of these outer membrane ...