The resistance of gastro-intestinal nematodes of small ruminants (sheep and goat) to benzimidazole anthelmintic drugs seems to be linked primarily to a single mutation in the isotype 1 b-tubulin gene. This study was carried out to investigate the origin and diversity of benzimidazole-resistance alleles in trichostrongylid nematodes. We sequenced a 550 bp fragment of the isotype 1 b-tubulin gene...

The Teladorsagia circumcincta P-glycoprotein-9 (Tci-pgp-9) gene has previously been implicated in multiple-anthelmintic resistance in this parasite. Here we further characterise genetic diversity in Tci-pgp-9 and its possible role in ivermectin (IVM) and multi-drug resistance using two UK field isolates of T. circumcincta, one susceptible to anthelmintics (MTci2) and the other resistant to most...

Resistance to benzimidazole (BZ) anthelmintics is common in ovine nematodes of economic importance. Single nucleotide polymorphisms (SNP) at three positions in the isotype 1 β- tubulin gene have been associated with BZ resistance and molecular tests for the detection of BZ resistance have been developed. In order to determine if such tests are practicable in Ireland the polymorphisms associated...

Parasitic nematodes cause chronic, debilitating infections in both livestock and humans worldwide, and many have developed multiple resistance to the currently available anthelmintics. The protective collagenous cuticle of these parasites is required for nematode survival and its synthesis has been studied extensively in the free-living nematode, Caenorhabditis elegans. The collagen synthesis p...

Assessments were made of the influence of several microclimatic variables on the availability of third-stage larvae of Ostertagia ostertagi, on pasture herbage. Variables most closely related to recovery of larvae from the lower herbage samples were: maximum air, mat and dung temperatures. Recovery of larvae from the upper portion of the herbage was closely correlated with dung temperature, sam...

Two experiments were carried out during summer and winter conditions to determine if Dictyocaulus vivipams infective larvae could survive on pasture during these seasons in Louisiana. Pairs of tracer calves were grazed in August and September on a pasture that was heavily contaminated in May and then left free of cattle during June-July. These tracers were not infected with D. vivipams, indicat...

Nematodes are one of the main impactors on health, welfare and productivity farmed animals. Teladorsagia circumcincta endemic throughout many sheep-producing countries, particularly in northern hemisphere, contribute to pathology economic losses seen farms. Control these nematode infections is essential heavily reliant chemotherapy (anthelmintics), but this has been compromised by development a...

Female Blackface lambs expected to exhibit genetic variability for resistance to gastrointestinal nematodes, were either exposed to continuous experimental infections of Teladorsagia circumcincta or were sham-dosed to monitor phenotypic responses to infection. As a measure of parasitism and host response, worm-eggs in faeces (faecal egg count, FEC) were counted over a 3-month period and worm bu...

During the past decade in South Africa there has been a continual increase in sheep of strains of gastrointestinal helminths resistant to the modern anthelmintics. Five strains of Haemonchus contortus are described in this paper. Despite the fact that 2 of the 5 strains were tested for susceptibility only to ivermectin, a total of 10 instances of resistance were found. Four of the 5 strains wer...