Ben-Ishai, Ruth (Israel Institute of Technology, Haifa), Michal Lahav, and Ada Zamir. Control of uracil synthesis by arginine in Escherichia coli. J. Bacteriol. 87:1436-1442. 1964.-It is shown that arginine affects uracil biosynthesis in Escherichia coli. The effect of arginine on uracil synthesis was analyzed by following the changes in the level of aspartate transcarbamylase, the first specif...

Fermentative growth via the arginine deiminase pathway is mediated by the enzymes arginine deiminase, carbamate kinase, and catabolic ornithine transcarbamylase and by a membrane-bound arginine-ornithine antiporter. Recently we reported the characterization of catabolic ornithine transcarbamylase and the corresponding gene, arcB, from Halobacterium salinarium (formerly Halobacterium halobium). ...

All metazoan cells in culture require either arginine or citrulline for growth (1, 2). The importance of arginine is emphasized by the fact that it is rapidly depleted from the medium in many primary and established cell strains (3). The L-929 fibroblasts maintained in this laboratory and shown to harbor a pleuropneumonia-like infection also demonstrate a.n elevated requirement for this amino a...

Experimental conditions were as follows: MgS04 , 10 pmoles; L-ornithine, 5 rmoles; glycylglycine, pH 7.5, 50 pmoles; NHIHC03, 5 Fmoles; ATP, 5 pmoles; phosphoenolpyruvate, 5 pmoles; ornithine transcarbamylase (beef liver) 17 units; phosphoenolpyruvate kinase, 513 Biicher units; frog liver carbamyl phosphate synthetase, 0.1 ml (0.825 mg); final volume, 1.00 ml; incubation for 15 minutes at 37”. ...