Tailing of DNA with terminal transferase is a technically demanding, but essential step in full-length cDNA cloning procedures*•^. It is also an essential step of a poly(dA) affinity labelling technique, which has been used to select families of partial restriction fragments on oligo(dT)cellulose^•*. In order to bypass the need for transferase in the latter technique, we made a simple modificat...

Rare earth mine tailing dumps are environmental hazards because tailing easily leaches and erodes by water and wind. To assess the influence of mine tailing on the geochemical behavior of rare earth elements (REEs) in soil, sixty-seven surface soil samples and three soil profile samples were collected from different locations near China's largest rare earth mine tailing. The total concentration...

Mining activities have resulted in a large volume of tailings containing certain proportion lost minerals, making them potential reprocessing opportunity, and information on mineral is often scarce. In this study, conceptual framework was established used to produce meaningful knowledge from the Nyamatete mine at HABATU Company Limited (an ASM Rwanda). Tailings particles were investigated based...

Non-specific tail sequences are often added to the 5'-terminus of primers to improve the robustness and overall performance of diagnostic assays. Despite the widespread use of tailed primers, the underlying working mechanism is not well understood. To address this problem, we conducted a detailed in vitro and in silico analysis of the enhancing effect of primer tailing on 2 well-established foo...

RFID (Radio-Frequency IDentification) is a key emerging technology for supply-chain monitoring and detection of counterfeit and grey-market goods. The most prevalent RFID tags are, however, simply “wireless barcodes,” themselves vulnerable to cloning and counterfeiting. While continuous monitoring can, in principle, detect cloning attacks, real-world supply chains often contain significant blin...

The rapid deterioration of the environmental situation comes mainly from pollution by industrial and municipal wastes in form landfilled wastes. only way out this threatening is urgent utilization all these as valuable raw materials alternative to traditional natural materials. This article describes detail result complete replacement with four wastes—from iron ore processing, cellulose pulp pr...

Workability is a key performance criterion for mining cemented tailing backfill, which should be defined in terms of rheological parameters such as yield stress and plastic viscosity. Cemented tailing backfill is basically composed of mill tailings, Portland cement, or blended cement with supplementary cement material (fly ash and blast furnace slag) and water, among others, and it is important...