The 165-kb chromosome of Epstein-Barr virus (EBV) is replicated by cellular enzymes only once per cell cycle in human cells that are latently infected. Here, we report that the human origin recognition complex, ORC, can be detected in association with an EBV replication origin, oriP, in cells by using antibodies against three different subunits of human ORC to precipitate crosslinked chromatin....

An open resonance cell (ORC) with finite-length cylindrical mirrors is suggested for making absolute measurements of complex permittivity in the case of stretched cylindrical specimens or liquid dielectrics in cylindrical containers. For H-polarization, we report ORC features simulated in rigorous electromagnetic terms using a twodimensional (2-D) model of an open resonator with cylindrical mir...

The eukaryotic pre-replication complex is assembled at replication origins in a reaction called licensing. Licensing involves the interactions of a variety of proteins including the origin recognition complex (ORC), Cdc6 and the Mcm2-7 helicase, homologues of which are also found in archaea. The euryarchaeote Archaeoglobus fulgidus encodes two genes with homology to Orc/Cdc6 and a single Mcm ho...

Air-cooled condensers are widely used for Organic Rankine Cycle (ORC) power plants where cooling water is unavailable or too costly, but they are then vulnerable to changing ambient air temperatures especially in continental climates, where the air temperature difference between winter and summer can be over 40 C. A conventional ORC system using a single component working fluid has to be design...

Huge amount of low-grade heat energy is discharged to the environment by vehicular engines. Considering the large number of vehicles in the world, such waste energy makes great impacts to our environment globally. Organic Rankine Cycle (ORC), which uses an organic fluid with a low boiling point working medium, is considered as the most promising technology to recover energy from low-grade waste...

Variety in histochemical characteristics of the olfactory receptor cells (ORC) was examined by immunohistochemistry for protein gene product 9.5 (PGP9.5) and calretinin, and by lectin histochemistry with Phaseolus vulgaris leucoagglutinin (PHA-L) in the olfactory epithelium (OE) of the barfin flounder (Verasper moseri). PGP 9.5 immunoreactivity was observed in the ORC situated in the upper thre...

Orc and Reo are two complementary approaches to the problem of coordinating components or services. On one hand, Orc is highly asynchronous, naturally dynamic, and based on ephemeral connections to services. On the other hand, Reo is based on the interplay between synchronization and mutual exclusion, is more static, and establishes more continuous connections between components (services). The...

The locally restricted recruitment of the multisubunit origin recognition complex (ORC) to eukaryotic chromosomes defines the position of origins of DNA replication. In budding yeast and metazoans the DNA binding activity of ORC is stimulated by ATP and requires an AAA+-type nucleotide binding domain in the largest subunit. Little else is known about the mechanisms behind the ATP requirement fo...

An interaction between the origin recognition complex (ORC) and Cdc6p is the first and a key step in the initiation of chromosomal DNA replication. We describe the assembly of an origin-dependent complex containing ORC and Cdc6p from Saccharomyces cerevisiae. Cdc6p increases the DNA binding specificity of ORC by inhibiting non-specific DNA binding of ORC. Cdc6p induces a concomitant change in t...

Cook and Misra’s Orc is an elegant language for orchestrating distributed services, able to cover e.g. van der Aalst’s workflow patterns. We aim to understand the key novel features of Orc by comparing it with variations of Petri nets. The comparison shows that Orc hides powerful mechanisms for name handling (creation and passing) and for atomic distributed termination. Petri nets with static t...