Protein supplementation may be an alternative to reduce the erosive potential of acidic drinks. The aim of this in vitro study was to evaluate the erosive potential of an orange juice modified by dietary proteins. A commercially available orange juice was added 0.2 g/L casein, 2.0 g/L ovalbumin and their combination. The juice with no additives and a commercially available calcium-modified juic...

It is well known that eating fruits and vegetables lowers the risk of chronic diseases such as heart disease and cancer. The question of what is/are the active ingredient(s) is still unresolved. The initial hypothesis was that the antioxidant vitamins were responsible. However, recently the polyphenols have been investigated since they have been found to have beneficial properties such as being...

Organic cation transporter 2 (OCT2) plays an important role in the renal clearance of endogenous and exogenous organic cations, including drugs and their metabolites. Citrus fruit juice interactions with the P-gp efflux transporter are well-established, but there is to date no study on the effects of citrus fruit juice on OCT2 function and expression. This paper evaluates the modulating activit...

Isotopic analysis was used to characterize authentic samples of orange juice (Citrus sinensis) from Brazil. Site specific natural isotopic fractionation nuclear magnetic resonance (SNIF-NMR) was used to determine deuterium/hydrogen ratios at the methyl [(D/H)I] and methylene [(D/H)II] sites of ethanol produced by fermentation of orange juice. Stable isotope ratio mass spectrometry (SIRMS) was u...

Different fortified foods have been used for the control of iron-deficiency anaemia in children. In Brazil, a low cost, abundant and culturally accepted food but not yet used for fortification, is orange juice. To evaluate the usefulness of fortified orange juice, 50 preschool children enrolled in a day-care centre in the town of Pontal, Southeast Brazil, received two flasks of 200 ml orange ju...

Diacetyl has been associated with the "buttermilk off-flavor" sometimes found in orange juice and in frozen concentrated orange juice. Bacteria of the Lactobacillus and Leuconostoc genera have been identified as causative agents of this type of spoilage (Hays, 1951; Hays and Riester, 1952; Murdock et al., 1952, 1953; and Hill et al., 1954). Growth rates of these spoilage organisms have been inv...

minimum inhibitory concentrations (mic) of some essential oils and citric acid were determined against two micro-organisms associated with spoilage of orange juice (saccharomyces cervisiae and leuconostoc mesenteroids) and against four unidentified microorganisms isolated from citrus surface and spoiled orange juice. mic for limonene were obtained less than 5% w/v aqueous against saccharomyces ...

This research describes a voltammetric approach to determine amounts of vitamin C (Ascorbic acid) in orange juice sample, using three screen printed electrode. The anodic currents of vitamin C were proportional to vitamin C concentration in the range of 0 – 10.0 mM with the limit of detection of 1.36 mM. The method was successfully employed with 2 μL of the working solution dropped on the elect...

Fruit juices each have very distinct organic acids profiles that can be used as fingerprints for establishing authenticity. A method has been developed, optimised and validated for measuring by capillary electrophoresis citric, isocitric, malic and tartaric acids as authenticity markers in orange juices, without any sample treatment other than dilution and filtration. Final conditions were phos...

A three-stage continuous fermentative colonic model system was used to monitor in vitro the effect of different orange juice formulations on prebiotic activity. Three different juices with and without Bimuno, a GOS mixture containing galactooligosaccharides (B-GOS) were assessed in terms of their ability to induce a bifidogenic microbiota. The recipe development was based on incorporating 2.75g...