Introduction The cryoconservation of pig embryos with intact zona pellucida would be a major advantage to control and then insure minimal risk of disease transmission during embryo transfer of genetic material. Moreover, international rules on embryo transport outside of the country origin require that only embryos with an intact zona pellucida may be exported [9]. A vitrification technique is ...

Cryostorage of porcine embryos in a closed pathogen-free system is essential for the maintenance and safeguard of swine models. Previously, we reported a protocol for the successful cryopreservation of porcine embryos at the blastocyst stage in 0.25 mL ministraws. In this experiment, we aimed at developing a protocol to apply the same concept for the cryopreservation of early-stage porcine embr...

A 65-year old, farm worker female with a six weeks right eye (RE) discomfort, tearing and progressive eyelid swelling was referred to our clinic. Past ophthalmic and systemic history was uneventful. She presented painless, considerable RE superior eyelid edema, bilateral best-corrected visual acuity of 0.9, normal ocular movement and pupil reflex. Slit lamp examination revealed conjunctival hyp...

Two hundred seventy-three crossbred steer calves were fed until slaughter (November through May, 189 days) in either semi-confinement (partial overhead shelter, 88 sq. ft. per steer, concrete pen surface) or conventional open lots (windbreak, 448 sq. ft. per steer, dirt surface with mound). Within each housing system, cattle were provided with no bedding or bedding in the form of oat straw or s...

Crop straw gasification technologies are the most promising biomass gasification technologies and have great potential to be further developed in China. However, the commercialization development of gasification technology in China is slow. In this paper, the technical reliability and practicability of crop straw gasification technologies, the economic feasibility of gas supply stations, the ec...