The slow-freezing method is widely used to freeze human oocytes, both for fertility preservation and in routine IVF programmes. Slow freezing damages some of the cell's structures, including the meiotic spindle (MS) and the zona pellucida (ZP). Polarized light microscopy was used to study the variations induced by slow freezing on the MS and the ZP of human oocytes and to analyse the relationsh...

Background: This study was set up to evaluate the effect of ovarian tissue slow freezing on in vitro growth and pattern of maturation genes expression in mouse preantral follicles encapsulated within alginate hydrogel. Materials and Methods: Ovaries of 12-14 days old female NMRI mice were randomly allocated into control and slow freezing groups. In slow freezing group, ovaries were equilibrated...

The newest innovation in assisted reproduction is oocyte cryopreservation, more commonly known as egg freezing, which has been developed a method of fertility preservation. Studies emerging from around the world show that highly educated professional women are turning to freezing their late thirties early forties, because they still searching for male partner with whom have children. For these ...

Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. ...

Abstract Compared to somatic or sperm cells, mammalian oocytes are much more sensitive cryopreservation mainly because of the large volume, cytoskeleton, and presence zona pellucida. Connections with surrounding cumulus cells also challenging preserve in immature oocytes. Besides genetic epigenetic information enclosed nucleus, contain organelles cytoplasmic factors that necessary for early emb...

STUDY QUESTION What is the nature of the relational status, reproductive choices and possible regret of a pioneer cohort of women that either considered or actually performed oocyte banking for anticipated gamete exhaustion (AGE)? SUMMARY ANSWER Only half of the women who banked oocytes anticipate using them in the future but the experience with oocyte banking is overwhelmingly positive, with...

Oocyte cryopreservation has recently emerged as an option for women to preserve their fertility for medical (e.g. treatable malignancy) or elective indications (e.g. advancing age). This report describes an IRBapproved study of over 200 oocyte cryopreservation cycles at one center. Patients presenting for oocyte cryopreservation (January 2005 to 2010) were analyzed for day 3 follicle stimulatin...

The techniques of vitrification of oocytes and the subsequent warming process being used today are now producing results far superior to the results that are obtained with slow-freezing techniques, and it would seem that this is the method of female fertility preservation that will be widely used in the near future. The reported success of the use of this method should stimulate a renewed debat...

This review presents some of the most noticeable aspects related with the oocyte cryopreservation procedures, emphasizing their evolution in the bovine, which points towards the critical points determining the reduced survival rates of female gametes to freezing and vitrification. Factors such as the maturation status, the cytoskeleton and membrane sensitivity, the role of the cumulus cells, th...

Success rates with in vitro fertilization (IVF) continue to improve as we gain insight into optimal culture conditions for gametes and embryos. New procedures such as in vitro egg maturation, preimplantation genetic testing, single embryo transfer, and oocyte freezing hold the promise of reducing the cost, inconvenience, and risks of IVF, as well as preserving future fertility. We provide an ov...