Chicken and human isolates of Campylobacter jejuni were used to provide oral challenge of day-old broiler chicks. The isolation ratio of the competing challenge strains was monitored and varied, depending upon the isolates used. A PCR-restriction fragment length polymorphism assay of the flagellin gene (flaA) was used to discriminate between the chick-colonizing isolates. Our observations indic...

Protection against group B meningococcal infection was examined using the chick embryo. 12-day-old embryos were challenged intravenously with various meningococcal strains. The chick embryo has an active reticuloendothelial system but lacks functional complement. In this model we found that protection against group B infection was primarily group specific. The group B polysaccharide antibody is...

When primitive erythroid cells from 5-day-old chick embryos are exposed to inactivated Sendai virus they do not undergo haemolysis but fuse with other cells by the normal process of cytoplasmic coalescence. In this way cells actively engaged in the synthesis of haemoglobin may be fused with others that are not. In heterokaryons formed by the fusion of such erythroid cells with cells from establ...

Homeobox-containing clones were isolated from a Lambda-ZAP cDNA library derived from somites of 2.5 day old chick embryos. The clones were identified by hybridization to the homeobox-containing Xenopus gene Xhoxl-A (1). Fourteen clones isolated from a screen of 3.3 xlO plaques of an amplified library contain homeobox sequences and some are highly conserved homologues of murine Hox genes. Sequen...

Sites binding monoiodinated-Bolton-Hunter-reagent-labelled substance P were solubilized from 1-day-old-chick brain membrane by using non-ionic detergents (1% digitonin/1% n-octyl glucoside) and a high concentration of NaCl (0.5 M). The solubilized preparation retained the pharmacological properties of the high-affinity binding sites found in the native membrane. The high density of specific bin...

The synergistic effect of Hemophilus influenzae suis and swine influenza virus in the pig can be reproduced by the inoculation of these agents on the chorioallantoic membrane of 9 to 10 day old chick embryos. Two strains of human influenza virus that were studied failed to substitute for the swine virus in the synergistic reaction. No loss of synergistic effect was noted when the swine influenz...

The titration curve for the virus of Eastern equine encephalomyelitis inoculated into the 10 day old chick embryo shows that the maximum increase in virus content continues until shortly before the generalized destruction of the embryo is apparent. This is followed by a stationary phase. Histological studies of infected embryos fail to demonstrate selective tissue destruction, and titrations sh...

  Objective(s): This study aimed to find out whether the chick cardiomyocyte micromass (MM) system could be employed to predict the teratogenecity of common environmental factors. Different multivitamins and over the counter drugs were used in this study.   Materials and Methods: White Leghorn 5-day-old embryo hearts were dissected and trypsinized to produce a cardiomyocyte cell suspension in D...

Case Description- A 20-day-old pigeon chick with left wing distal to the carpal joint into left flank presented to our avian medicine and surgery clinic. Clinical Findings-The bird had a mal-shaped wing structure, whereas the axial skeleton, thoracic and pelvic limbs skeleton were normal radiographically and clinically, excluding the left wing. The only clinical sign observed was recumbency. Tr...

Background: The chick embryo is a classical model to study embryonic development. However, most researchers have not studied the effect of embryonic manipulation on chick hatchability. To determine the effect of egg orientation and type of sealing film on the hatchability of cultured embryos, and to determine the fate of adulte stem cells injected into the blastoderm. Materials and Methods: Win...