to investigate the genetic diversity of 21 rapeseeds (brassica napus l.), 20 semi-random isj and ssr primers were used. primers produced a total number of 158 (81.5%) polymorphic bands (51 ssr and 107 isj bands). average numbers of polymorphic bands for isj, ssr and combined of them were 10.7, 5.1 and 7.9, respectively. pic values ranged from 0.0150 for specific primer o110d08 to 0.3420 for sem...

We employed RAPD and sequence-related amplified polymorphism (SRAP) markers to evaluate polymorphisms in 15 tomato (Solanum lycopersicon) genotypes that were obtained from a tomato breeding program. Four local tomato genotypes selected from the Sanliurfa province (Southeastern Anatolia Region of Turkey), 10 heat-tolerant tomato genotypes, received from the Asian Vegetable Research and Developme...

Genetic relationships of 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its two wild relatives (C. albicans and C. lineatus) from different parts of the India were analysed using 22 random amplified polymorphic DNAs (RAPDs) primers and 10 inter simple sequence repeats (ISSRs) primers. Twenty two RAPD primers yielded 151 polymorphic markers (71.2%) with an average of 6.8 polymorphic b...

RAPD-PCR genetic markers were used to assess variation in wheat plants and connections among six genotypes. Four random primers produced 140 DNA fragments, averaging 6.7 identifiable bands per primer. Among the genotypes, 85 pieces (44.64 percent) polymorphic. Several RAPD marker had distinct signify recurrence patterns that thing differently amongst germplasm of groupings. Within-community acc...

ziziphora tenuior, named kakooti in farsi, is one of the aromatic and medicinal plants of a wide distribution in iran. in this study, rapd molecular markers, (by utilization of 16 primers) was applied to determine the genetic diversity of 39 samples of ziziphora tenuior from 21 geographical regions in iran. a number of 100 polymorphic bands out of 110 amplified ones was produced and analyzed th...

Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 R...

The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice for all individuals, a few randomly chose...

Chicken meat is a valuable source of protein and consumption of it continues to rise day to day. The aim of the present study was to evaluate the genetic homogeneity of Listeria monocytogenes (L. monocytogenes) isolates obtained from chicken carcasses and human. Random Amplification of Polymorphic DNA (RAPD) PCR with three different primers were used to analyze the 31 L. monocytogenes isolates ...

| Poultry and poultry products are the most economical protein sources for the ever-increasing human population and to meet the future food security. While these are promising food sources, the importance of genetically improved avian species in the breeding programme has increased significantly. In this study, we applied randomly amplified polymorphic DNA (RAPD) methodology to elucidate geneti...