1. Isolated blood cells, when placed for incubation in a plasma substratum, are capable of constructing highly organized, tubular processes that project from the explanted cell mass into the surrounding medium. 2. The tubular structures have fibrillar walls that may be covered, eventually, by a membranous layer of leukocytes. Their lumina contain blood cells suspended in fluid. 3. The formation...

Abstract Corydalis saxicola Bunting, a medicinal herb known for alkaloids, grows mainly in Guizhou province and northwest Guangxi of China. In this study, the first time plant, an efficient adventitious shoot proliferation regeneration system via organogenesis from leaf petiole explants was established. Cytokinins, including 6-benzyladenine (BA) thidiazuron (TDZ), induced callus then organogene...

A 65–yrs-old man referred for an ocular discomfort and red eye. The clinical diagnosis was leech in the left eye and its species confirmed as being Limnatis nilotica. Ocular leech infestation should be considered in patients with a history of swimming or washing their face in streams and lakes. Attention should also be given to ocular leech infestation in the differential diagnosis of ocular tr...

The world's most expensive wood, sandalwood (Santalum album L.), requires a stringent mass-propagation technique to prevent future scarcity. Plant tissue culture is an efficient method that regenerates the whole plant from single cell on hormone-based growth medium. To efficiently regulate formation of plants, it important understand developmental organogenesis pathways (i.e., direct and indire...

Background: Tolmetin (Tol) is a pyrrole acetic acid derivative that acts as a highly effective topical anti-inflammatory agent. Topical administration of Tol is effective for the management of ocular inflammations.The aim of this study was to formulate and improve a topical ophthalmic delivery system of Tol. Materials and methods: In the present study, ocular inserts of Tol were prepared using ...

In many animals, germ cells migrate to the gonad to assemble into a functional organ. Recent work in Drosophila has built a picture of the gene activities that specify the gonad and allow it to attract germ cells, and has led to the identification of a gene, columbus, that may encode the attractive factor.

temporal coordination of numerous biomolecular interactions. Approaches geared toward elucidating the molecular and cellular basis of kidney development and branching morphogenesis have focused on the study of discrete molecules and signaling pathways by using genetic and in vitro approaches (reviewed in Refs. 11 and 71), more recently combined with microarray studies (66, 74, 75). To date, int...

The recognition that lymphocytes existed in different varieties and that lymphoid organs were important for their differentiation greatly influenced immunological research. The growing awareness that started in the mid-fifties of the previous century has shifted the emphasis of immunology from a molecular, mostly serological science to the cell-oriented modern immunology of today. Matters such ...

Transcription factors drive organogenesis, from the initiation of cell fate decisions to the maintenance and implementation of these decisions. The Drosophila embryonic salivary gland provides an excellent platform for unraveling the underlying transcriptional networks of organ development because Drosophila is relatively unencumbered by significant genetic redundancy. The highly conserved FoxA...