In this paper, we describe a peptide library designed by computational modelling and the selection of two peptide sequences showing affinity towards the mycotoxin, ochratoxin A (OTA). A virtual library of 20 natural amino acids was used as building blocks to design a short peptide library against ochratoxin A template using the de novo design program, LeapFrog, and the dynamic modelling softwar...

To determine whether ochratoxin is produced by species of Aspergillus other than A. ochraceus, 44 strains representative of the nine species composing the A. ochraceus group were grown on both pearled wheat and cracked corn in duplicate shaken flasks at 28 C for 5 days. Rice was a poor substrate for production of ochratoxin. Molded substrates were extracted with acetonitrile-water; partially pu...

In this paper, we developed a simple method to detect fungi toxin (ochratoxin A) produced by Aspergillus Ochraceus and Penicillium verrucosumm, utilizing graphene oxide as quencher which can quench the fluorescence of FAM (carboxyfluorescein) attached to toxin-specific aptamer. By optimizing the experimental conditions, we obtained the detection limit of our sensing platform based on bare graph...

Ochratoxin A (OTA)-a toxin produced by Aspergillus carbonarius, Aspergillus ochraceus, and Penicillium verrucosum-is one of the most-abundant food-contaminating mycotoxins. To avoid the risk of OTA consumption for humans and animals, the rapid detection and quantitation of OTA level in different commodities are of great importance. In this work, an impedimetric immunosensor for ochratoxin A (OT...

The extraction of ochratoxin A from meat products is generally carried out using chlorinated organic solvents, such as chloroform or methyl chloride, acidified with hydrochloric or o-phosphoric acid. In this study, an innovative method was developed to extract ochratoxin A from pork and dry-cured ham samples. The method was based on an enzyme-assisted extraction with pancreatin in phosphate buf...

G. A. SANSING, E. B. LrLLEHOJ, R. W. DHROY and M. A. MILLER. Synergistic toxic effects of citrinin, ochratoxin A and penicillic acid in mice. Toxicoll 14, 213-220, 1976.-The LD50'S in mice of citrinin, ochratoxin A, and penicillic acid injected intraperitone,"lIy were 89,22, and 100 mg per kg of body weight, respectively. Paired combinations of the mycotoxins, citrinin: ochratoxin A (Cl:OA), oc...

Mycotoxins are toxic secondary fungal metabolites. A large variety of mycotoxins have been described, but only a minority of them occur regularly in feed and food items under normal conditions and pose a risk to feed and food safety. The important moulds producing toxins during storage belong to the genera of Penicillium, or Aspergillus. The most important storage toxins are probably ochratoxin...

A manuscript in the journal recently cited experimental rat data from two manuscripts to support plausibility of a thesis that ochratoxin A might be a cause of human testicular cancer. I believe that there is no experimental evidence that ochratoxin A produces testicular cancer in rats or mice.

Mould and mycotoxin contamination in food OTA toxicity Ochratoxin A producers Occurrence of Ochratoxin A Effect of water activity on OTA production OTA destruction during roasting of coffee Levels and distribution of OTA in green coffee (contamination, survey) Effect of processing and handling on OTA content of green coffee Effect of caffeine on OTA production Socioeconomic impact of coffee pro...

Ochratoxin A (OTA) exposure via ingestion and inhalation has been described in the literature to cause kidney disease in both animals and humans. This paper reviews Ochratoxin A and its relationship to human health and kidney disease with a focus on a possible association with focal segmental glomerulosclerosis (FSGS) in humans. Prevention and treatment strategies for OTA-induced illness are al...