Background: The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development however, their role on metabolic pathway is not well understood. Methods: In the present study, the effect of different...

This study describes the physical stability and optimization of nutrient components for an extracellular protease produced by Bacillus strains isolated from fruits and vegetable waste, Lucknow, India. The isolated proteases could hydrolyze various native proteinaceous substrates such as bovine serum albumin, casein, skim milk, but not the gelatin. The strain JX416854 and isolate 10 yielded maxi...

Fourier Transform Infrared (FT-IR) spectroscopy is a robust method for macromolecular analysis and differentiation of microorganisms. However, most studies are performed in controlled conditions and it is unclear whether this tool is appropriate for the identification of eukaryotic microalgae species from variable environments. In order to address this, nine closely-related species of marine an...

Increasing the adoption of cycling is crucial for achieving more sustainable urban mobility. Navigating larger cities on a bike is, however, often challenging due to cities’ fragmented cycling infrastructure and/or complex terrain topology. Cyclists would thus benefit from intelligent route planning that would help them discover routes that best suit their transport needs and preferences. Becau...

The effectiveness of ocean-colour data assimilation in providing robust biological-parameter estimates for basin-scale ecosystem models is investigated for a phytoplankton-zooplankton-nutrient model using North Atlantic satellite chlorophyll data. The model is forced by annual cycles of mixed-layer depth, day length, photosynthetically available radiation and a temperature-dependent phytoplankt...

Current industry practices for large-scale mammalian cell cultures typically employ a standard platform fed-batch process with fixed volume bolus feeding. Although widely used, these processes are unable to respond to actual nutrient consumption demands from the culture, which can result in accumulation of by-products and depletion of certain nutrients. This work demonstrates the application of...

We report results from a series of studies coevolving players for simple Rock–Paper–Scissors games. These results demonstrate that “Current Individual versus Ancestral Opponent” (CIAO) plots, which have been proposed as a visualization technique for detecting both coevolutionary progress and coevolutionary cycling, suffer from ambiguity with respect to an important but rarely discussed class of...

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Diet affects both lifespan and reproduction [1-9], leading to the prediction that the contrasting reproductive strategies of the sexes should result in sex-specific effects of nutrition on fitness and longevity [6, 10] and favor different patterns of nutrient intake in males and females. However, males and females share most of their genome and intralocus sexual conflict may prevent sex-specifi...