Mouse hepatitis virus (MHV) belongs to the group II coronaviruses. The virus produces nine genes encoding 11 proteins that could be recognized as structural proteins and nonstructural proteins and are crucial for viral RNA synthesis. The nucleocapsid (N) protein, one of the structural proteins, interacts with the 30.4 kb virus genomic RNA to form the helical nucleocapsid and associates with the...

abstract in october 2009, in one of the alstroemeria (alatroemeria sp.) greenhouses, located in khorasan razavi province, tospovirus-like symptoms (necrosis) were observed on stems, petioles, flowers and leaves of some plants. sap extracts from these plants were mechanically inoculated to indicator plants. necrotic local lesions were observed on petunia hybrida and chenopodium quinoa. necrotic ...

Persistent viral infections can render host cells resistant to superinfection with closely related viruses by largely uncharacterized mechanisms. We present evidence for superinfection exclusion in brains of Borna disease virus (BDV)-infected rats and in persistently infected Vero cells, and we suggest that acquired resistance to BDV is due to unbalanced intracellular levels of viral nucleocaps...

We report a comparative study in which a single-molecule fluorescence resonance energy transfer approach was used to examine how the binding of two families of HIV-1 viral proteins to viral RNA hairpins locally changes the RNA secondary structures. The single-molecule fluorescence resonance energy transfer results indicate that the zinc finger protein (nucleocapsid) locally melts the TAR RNA an...

Antibody response against nucleocapsid and spike proteins of SARS-CoV-2 in 11 persons with mild or asymptomatic infection rapidly increased after infection. At weeks 18-30 diagnosis, all remained seropositive but protein-targeting antibody titers declined. These data may be useful for vaccine development.

Chicken infectious bronchitis virus (IBV) causes severe respiratory and renal dysfunction in chickens. In the present study, we used reverse transcription-polymerase chain reaction (RT-PCR) to amplify the nucleocapsid (N) protein gene of strain Massachusetts (Mass) H120 of IBV that is commonly used in the vaccine production in Iran. The PCR product with the expected size of 1.2 kb was cloned in...

Significance We present a generic methodology that extracts structural data from living, intact cells for any protein of interest. Application this to different viral proteins resulted in significant cross-link sets revealed the connectivity within their structures. Importantly, we show these are detailed enough enable integrative modeling full-length sequence. Consequently, report global organ...