A study of histopathological changes due to the infection of Helicoverpa armigera Nuclear Polyhedrosis Virus (HANPV) in the third instar larvae of H. armigera at 24, 48, 72 and 96 hour interval was carried out in the laboratory. The histopathalogical sequential changes in the post infected H. armigera revealed the presence of infected area in the midgut epithelium at 72 hpi (hour post infection...

The isoelectric points of three baculoviruses, Trichoplusia ni nuclear polyhedrosis virus (NPV), T. ni granulosis virus, and Spodoptera littoralis NPV were identified by cell electrophoresis. At neutral pH polyhedra were negatively charged. T. ni NPV polyhedra were reacted with a number of reagents which could potentially attach to or degrade their surface structure. This gave information on th...

The gene encoding a 37-kDa glycoprotein (gp37) of Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus (OpMNPV) was located and sequenced. gp37 of OpMNPV was found to have 62 and 37% amino acid sequence identity with gp37 of Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) and with a protein reported to be a component of occlusion bodies from Choristoneu...

Genomic expression of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) was studied. Viral specific transcripts expressed in cell culture at various times from 2 through 72 h postinfection were identified and their genomic origins mapped through Northern analysis. Sixty-five distinct transcripts were identified in this analysis. Most viral transcripts were expressed lat...

Expression of the baculovirus major envelope glycoprotein gene (gp64) is regulated by transcription from both early and late promoters. To characterize the early promoter and identify sequences involved in the regulation of gp64 early transcription, promoter-reporter gene fusions were generated from the Orygia pseudotsugata nuclear polyhedrosis virus gp64 promoter and were analyzed by transient...

Spodoptera litura dapat menyebabkan kehilangan hasil hingga 80% bahkan gagal panen. Nuclear Polyhedrosis Virus merupakan agensi hayati dari kelompok patogen serangga famili Baculoviridae yang banyak digunakan sebagai agens dan (SlNPV) salah satu pemanfaatan NPV efektif dalam mengendalikan litura. Kendala penggunaan SlNPV yaitu tidak tahan terhadap sinar matahari sehingga perlu bahan pelindung a...

The gene encoding Bacillus anthracis protective antigen (PA) was modified by site-directed mutagenesis, subcloned into baculovirus and vaccinia virus plasmid transfer vectors (pAcYM1 and pSC-11, respectively), and inserted via homologous recombinations into baculovirus Autographa californica nuclear polyhedrosis virus or vaccinia virus (strains WR and Connaught). Expression of PA was detected i...