نتایج جستجو برای: nptii
تعداد نتایج: 262 فیلتر نتایج به سال:
Genetic screening identified a suppressor of ros1-1, a mutant of REPRESSOR OF SILENCING1 (ROS1; encoding a DNA demethylation protein). The suppressor is a mutation in the gene encoding the largest subunit of replication factor C (RFC1). This mutation of RFC1 reactivates the unlinked 35S-NPTII transgene, which is silenced in ros1 and also increases expression of the pericentromeric Athila retrot...
Many virulence genes in plant bacterial pathogens are coordinately regulated by "global" regulatory genes. Conducting DNA microarray analysis of bacterial mutants of such genes, compared with the wild type, can help to refine the list of genes that may contribute to virulence in bacterial pathogens. The regulatory gene algU, with roles in stress response and regulation of the biosynthesis of th...
Large quantities of DNA sequence information about plant genes are rapidly accumulating in public databases, but to progress from DNA sequence to biological function a mutant allele for each of the genes ideally should be available. Here we describe a gene trap construct that allowed us to disrupt transcribed genes with a high efficiency in Arabidopsis thaliana. In the T-DNA vector used, the ex...
Pollen tube pathway mediated transformation is most favorable genotype-independent approach and has been widely used for the production of transgenic crops all over the world. We used this technique to transform Zheda B, an upland cotton, by two different vectors i.e. pCAMBIA1301 with hpt gene for hygromycin resistance and pCAMBIA 2301 having nptII gene for kanamycin resistance. A preliminary e...
To elucidate the mechanism for intermolecular homologous recombination in plants we cotransformed Nicotiana tabacum cv Petit Havana SR1 protoplasts with constructs carrying different defective derivatives of the NPTII gene. The resulting kanamycin resistant clones were screened for possible recombination products by PCR, which proved to be a valuable technique for this analysis. Our results sho...
Levels of induced homologous recombination between chromosomal repeats in plant somatic cells were examined. Transgenic plants of Nicotiana tabacum hemi- or homozygous for pairs of deletion derivatives of the neomycin phosphotransferase (nptII) marker gene integrated at a single genomic locus were produced. Homologous recombination within the overlapping parts of the nptII gene restored the fun...
هدف: طراحی و تهیه سازه ژنی مناسب و انتقال آن به گیاه توتون و بررسی گیاهان تراریخت حاصله بوده است.مواد و روشها: سازه اختصاصی بذر حاوی پیشبرنده Napin، توالی Ω ، ژن GUS و توالی SAR در پلاسمید pBI121 طراحی و تهیه و در باکتری E. coli تکثیر شد. ریزنمونههای برگی توتون با آگروباکتریوم سویه LBA4404 بهروش استاندارد تلقیح شدند. گزینش جوانههای باززایی شده در محیط...
Abstract Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production Background: Selectable marker gene (SMG) systems are critical for generation of transgenic crops. Transgenic crop production without using SMG is not economically feasible. However, SMGs are non-essential once an intact transgenic plant has been established. Eli...
An efficient transformation protocol based on kanamycin selection was developed for Agrobacterium-mediated transformation of maritime pine embryonal masses. The binary vector pBINUbiGUSint, which contained neomycin phosphotransferase II (nptII) as a selectable marker gene and β -glucuronidase (uidA) as a reporter gene, was used for transformation studies. Different factors, such as embryogenic ...
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