Post-nodal pieces of the stage-4 chick blastoderms were transected 0-6 mm posterior to Hensen's node and cultured in vitro with and without chicken-heart RNA. After 24 h the explants were fed daily with fresh egg-extract medium for 4 days. On the 4th day pulsating heart was found in the RNA-treated but not in the untreated series. Histological examination revealed that cell differentiation othe...

In the present investigation, the influence of different forms of cytokinins, auxins and polyamines were tested for mass multiplication and regeneration of cotton. Initially, for the identification of effective concentration for multiple shoot induction, various concentrations of BAP, Kin and 2iP along with IAA and NAA were tested. Among tested concentrations, media fortified with MS salts; B5 ...

Chrysanthemum nodal explants were cultured on 1⁄2 X MS medium containing different combinations of growth hormones. A reasonable callus formed on explants when MS contained 0.5 mg. cm each of BAP and NAA after about 3 weeks. Similar results were obtained on MS + 0.5 mg. cm BAP + 0.1 mg. cm NAA or 1.0 mg. cm BAP + 0.1 mg. cm NAA. A maroonish embryogenic callus was formed on MS containing BAP onl...

Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was fou...

Healthy multiple shoot regeneration was observed from petiole-attached cotyledon (PC) explants of CVL-1 on MS containing 0.2 mg/l BAP and 1.0 mg/l IAA. On the other hand, the best response for multiple shoot regeneration in CVE-3 was obtained when the same explants were cultured on MS with 2.5 mg/l BAP and 0.5 mg/l NAA. However, the same explant of D-154 was found to show less responsive compar...

Withania somnifera (L.) Dunal is high demanding valuable medicinal plants which have pharmaceuticals properties, known for its with anolides. Nodal segment explants of W. somnifera when cultured on MS (Murashige and skoog’s, 1969) medium horizontally containing different concentration(1-3mg/l) of 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ) produced multiple shoots on both axil...

An efficient protocol for in vitro clonal propagation of Smilax zeylanica was developed using nodal and shoot tip segments from field grown plants. Nodal explants performed better than when two distinct were tested proliferation. The best results proliferation achieved with MS medium supplemented BAP Kn alone or combination NAA IBA at various concentrations. On containing 4.0 ?M BAP, the greate...