Two murine monoclonal antibodies, IIG5 (IgG3) and IVE8 (IgG2a), that bind to Pseudomonas aeruginosa type a flagella and type b flagella, respectively, were prepared by conventional hybridoma methodology. Specificity of each monoclonal antibody for type a or type b flagella was demonstrated by enzyme-linked immunosorbent assay, indirect immunofluorescence, and immunoblotting. The percentage of P...

background infections caused by shigella are a major cause of diarrheal disease in the developing and developed countries. the present study was conducted to apply and evaluate arbitrarily primed pcr (ap-pcr) for investigation of genetic relatedness among the strains of shigella sonnei isolated from cases of acute diarrhea in tehran. patients and methods totally, 60 s. sonnei strains isolated f...

a survey was carried out in five provinces of iran (kerman, sistan and baluchestan, hormozgan, khorasan and yazd) for the presence of alfalfa mosaic virus (amv) sero-types in alfalfa during 2002 to 2003. the number of samples collected was 250, represent-ing the diversity and geographical distribution of amv in these areas. diagnosis was car-ried out using polyclonal (pabs) and monoclonal (mabs...

Methods to detect Taura syndrome virus (TSV) were assessed for their ability to detect the virus during chronic phase infection in the Pacific white shrimp Penaeus vannamei. In situ hybridization (ISH), immunohistochemistry (IHC) using monoclonal antibody 1A1, conventional RT-PCR and real-time quantitative (q)RT-PCR were compared using shrimp sampled over 60 wk following experimental TSV infect...

BACKGROUND Three human polyomaviruses have been classified as probable (Merkel cell polyomavirus) or possible (BK and JC polyomaviruses) carcinogens, but few epidemiologic studies have examined associations between this growing class of viruses and risk of non-Hodgkin lymphoma (NHL). METHODS Associations between polyomavirus antibodies and NHL incidence were examined using data from the Ameri...

A murine monoclonal antibody specific for glycoprotein (GP)IIIa was prepared by immunization with a GPIIb- and GPIIIa-enriched Triton X-114 extract of platelet membranes. This antibody, designated AP-3, was shown by indirect immunoprecipitation to react solely with GPIIIa derived from either P1A1-positive or -negative individuals. The epitope on GPIIIa recognized by AP-3 is expressed on dissoci...

In recent years, Salmonella enterica serovar Enteritidis has been a primary cause of human salmonellosis in many countries. The major objective of this study was to investigate genetic diversity among Salmonella Enteritidis strains from different origins (food and human) by Enterobacterial Repetitive Intergenic Consensus (ERIC) -PCR, as well as to assess their plasmid profiling and antimicrobia...

rhizoctonia zeae causes root rot and sheath blight on corn (zea mays) and several other gramineous plants. in order to determine the genetic diversity of the r. zeae population in mazandaran and golestan provinces, samples were collected from cultivatd soils of both provinces in 2011 and 2012.the identity of isolates as r. zeae were verified by amplification and sequencing of the 18s and 28s rd...

Considering the different clinical symptoms of leishmaniasis in Iran, and the wide distribution of its agent and the little information in this regard, this study was performed to characterize the responsible variants using monoclonal antibodies. A total of 156 isolates of Leishmania from patients with cutaneous leishmaniasis and one isolate from rat were characterized using the standard monoc...