1. An acid ninhydrin reagent was found to react specifically in forming a pink product (E(max.) 560mmu) with cysteine. 2. The method was highly sensitive for the determination of cysteine (in 28.0x10(3)). Homocysteine, glutathione, proline, ornithine and other naturally occurring amino acids tested did not give a similar reaction. 3. The reaction product was stable for at least 3-4hr. at room t...

1 Harding, V. J., and Warneford, F. H. S., J. Biol. Chem., 1916, xxv, 319. 2 Dakin, H. D., and Dudley, H. W., J. Biol. Chem., 1913, xv, 127. 3 Neuberg, C., Biochem. Z., 1913, lvi, 500. 4 Ruhemann, S., J. Chem. Sot. 1910, xcvii, 2026. 5 In this and the preceding paper triketohydrindene hydrate has sometimes been written as the triketone for the sake of simplicity in explaining the decompositions...

A facile and efficient one-pot synthesis of pyrrolidines and dispiro pyrrolidines/pyrrolizidines has been accomplished by the 1,3-dipolar cycloaddtion reaction of azomethine ylides generated insitu from paraformaldehyde/ninhydrin and secondary amino acids with electron-deficient dipolarophiles in good yield. The reaction proceeded with high regio and stereoselectivity. The products have been ch...

Biochemical methods originally designed for mammalian tissues are not, in all instances, directly adaptable to tissues from other species, such as fish. Consideration must be given to the biochemical profile of the research animal to which an analytical method is to be applied. In particular, when adapting a method for an amino acid such as phenylalanine from mammalian serum to fish serum, one ...

The reaction of 2,4,6-trinitrobenzenesulfonic acid (TNBS) with amino acids was investigated by a static system to develop optimum conditions for application to automated amino acid chromatography. TNBS was found to be a very simple and sensitive reagent, easier to use than ninhydrin. The reaction occurs quite rapidly at room temperature, eliminating the requirement of a heating bath. The major ...

An efficient acid induced rearrangement of a tetrahalo-7,7-dimethoxybicyclo[2.2.1]heptenyl system leading to substituted indenones is reported. This domino reaction involves dehydration, olefin isomerization, ketal hydrolysis, [3,3]-sigmatropic rearrangement and dehydrohalogenation. The resultant vicinal dihalo olefin moiety in the efficiently generated indenone derivatives was utilized to tran...

We prepared a brand new molecule in one step for the synthesis of bis-indolylindane-1,3-dione and indan-1,3-diones from the reaction of ninhydrin and 3 substituted/unsubstituted indoles using [Hbim]BF4 ionic liquid in excellent yields. The method was also used for the synthesis of novel indene-1,3(2H)-denies derivatives.

Ninhydrin reagents are currently used for amino acid determination. However, ninhydrin reacts with amino groups of amino acids and other components of palmar sweat to develop Ruheman’s purple. Therefore, it was used on a large scale to fingerprint development. Another recent application of ninhydrin is related to trace cyanide detection and determination. However, this simple compound may have ...