Intracellular pH (pHi) was measured with a pH-sensitive microelectrode in voltage-clamped sheep cardiac Purkinje fibers while tension was simultaneously measured. All solutions were nominally CO2/HCO3 free and were buffered with Tris. The addition of NH4Cl (5-20 mM) produced an initial intracellular alkalosis that was associated with an increase of twitch tension. At the same time, a component ...

We used sporogenous mutants of Dictyostelium discoideum to investigate the mechanism(s) by which exogenous NH4Cl and high ambient pH promote spore formation during in vitro differentiation. The level of NH4Cl required to optimize spore formation is correlated inversely with pH, indicating that NH3 rather than NH4+ is the active species. The spore-promoting activity of high ambient pH (without e...

The purpose of this investigation was to determine the role of enzyme adaptation in the response of ammonia excretion to acidosis in developing rats. The response of renal ammonia excretion was low in infant rats (7-12 days old) following administration of a single dose of acidifying salt (5 mmol NH4CL/kg). However, repeated administration (2 times daily) of the salt increased ammonia excretion...

Indium oxide (In2O3) nanocrystals (NCs) have been obtained via atmospheric pressure, chemical vapour deposition (APCVD) on Si(111) via the direct oxidation of In with Ar:10% O2 at 1000 C but also at temperatures as low as 500 C by the sublimation of ammonium chloride (NH4Cl) which is incorporated into the In under a gas flow of nitrogen (N2). Similarly InN NCs have also been obtained using subl...

Isolated hepatocytes prepared from sheep fed a basal diet (bromegrass hay-corn, 50:50 wt/ wt, as-fed basis) with or without urea were used to determine the effects of added ammonia (as NH4Cl) and propionate on the partitioning of C from 1.25 mM L-[1-14C]alanine between oxidation and gluconeogenesis, and the flux of 15N from 1.25 mM L-[15N]alanine to [14N15N]urea and [15N15N]urea. Hepatocyte sus...

Response surface methodology (RSM) was employed to study the effects of four medium components (L-glutamic acid, citric acid, glycerol and NH4Cl) on the production of poly-γ-glutamic acid (PGA) in flask cultures by Bacillus licheniformis CCRC 12826 (ATCC 9945) with initial medium pH 6.5. The optimal PGA yield (35.34 g/L, average of four repeats) was appeared at the region where the respective c...

in this research, aluminizing packs containing naf and nh4cl activators were used for aluminizing of pure titanium samples at 1100 ºc. the results showed that the layers of tial3, tial2, γ-tial, α2-ti3al, and α-solid solution are formed on titanium samples in both cases. however, the weight gain and the thickness of tial3 layer in treated samples with nh4cl activator are greater than those when...