An approach coupling signal processing and partial least-squares regression analysis (PLS) is described in which raw spectral data are processed with a multiple band-pass Ž lter and the Ž ltered spectra are used in a PLS to build a calibration model for the analyte of interest. The multiple band-pass Ž lter is speciŽ cally designed for a desired analyte based on the Fourier frequency characteri...

A simple analytical procedure is proposed for simultaneous determination of three common dyes (Basic Blue 9, Brilliant Blue E-4BA, and Reactive Blue 2) in natural waters without prior separation of the solutes. A popular chemometric method, partial least squares regression PLS-1, was effectively applied for spectral resolution of a highly overlapping system. At the best modeling conditions, mea...

Near-infrared measurements of glucose in human plasma are performed using a custom, rapid, high-throughput filter-based spectrometer covering a spectral range between 2080 and 2315 nm. Quality of the measured glucose signals is quantified through the use of two figures of merit: selectivity and limit of detection. Selectivity measures the uniqueness of the glucose spectrum from among the interf...

Line-dependent analyte signal suppression/enhancement by 18 common matrix elements was simultaneously investigated for MIP-OES. Li caused the strongest effect, even concentrations as low 20 mg L −1 .

Catalysis-based signal amplification makes optical assays highly sensitive and widely useful in chemical and biochemical research. However, assays must be fine-tuned to avoid signal saturation, substrate depletion and nonlinear performance. Furthermore, once stopped, such assays cannot be restarted, limiting the dynamic range to two orders of magnitude with respect to analyte concentrations. In...

We leveraged chemical-induced changes to microwave signal propagation characteristics (i.e., S-parameters) characterize the detection of aliphatic alcohol (methanol, ethanol, and 2-propanol) vapors using TCNQ-doped HKUST-1 metal-organic-framework films as sensing material, at temperatures under 100 °C. show that sensitivity depends on oxidation potential analyte, impedance setup analyte-loading...

A naturally occurring aptazyme, the glmS ribozyme, is adapted to an assay for glucosamine 6-phosphate, an effector molecule for the aptazyme. In the assay, binding of analyte allosterically activates aptazyme to cleave a fluorescently labeled oligonucleotide substrate. The extent of reaction, and hence analyte concentration, is detected by either fluorescence resonance energy transfer (FRET) or...

in this paper the author considers a general method, based on time domain samples for spectral manipulation of time limited signals. first, the original signal is divided into some frames in the time domain. then, by presenting a suitable theoretical and computational algorithm, and using a method for improving the speed of convergence, we find the local bandwidth of each frame; thereby, each f...

Surface plasmon field-enhanced fluorescence energy transfer is employed for sensitive optical readout of a reversible hairpin aptamer assay that is suitable for continuous monitoring of low-molecular-weight chemical analytes. A hairpin aptamer specific to adenosine and adenosine triphosphate with Alexa Fluor 647 fluorophore attached to its 5' end was anchored via 3' end thiol to a gold thin fil...

We present the design, tailoring, and physicochemical properties of a multicomponent polyethylene glycol (PEG)-based polymer doped with spherical nanoparticles modified lipoic acid derivative cyclodextrin its application for electrochemical sensing ferrocene ibuprofen at low concentrations. Each components this hybrid material has specific that can be useful preparation sensors characterized by...