mycoplasma gallisepticum is the most important and infectious mycoplasmosis. it is caused lots of economic losses for poultry's industry of iran. the target of this study is comparison of culture and nested pcr techniques to detect mycoplasma gallisepticum infection of chicken’s from industrial farms in kerman province of iran. 88 isolates received from industrial farms of kerman province of ir...

The definitive diagnosis ability of IgM ELISA, nested RT-PCR and real-time quantitative PCR (qPCR) was evaluated for Chikungunya diagnosis using 180 clinical samples. Real-time qPCR showed a higher sensitivity (88.3%) for Chikungunya diagnosis in the early stages of infection, while IgM ELISA proved sensitive for the late stages of illness (81.8%). The results suggest that the application of bo...

background: ovine babesiosis is the most important haemoparasitic tick-borne disease of small ruminants in iran caused by babesia ovis, b. motasi, and b. crassa . the aim of this study was to characterize the species of ovine babesia species isolated from different geographical region of iran. methods: one hundred fifty four blood samples collected from animals, which demonstrated the pale muco...

The clinical value of an in-house cytomegalovirus nested polymerase chain reaction (CMV-PCR) and a commercial molecular assay hybrid capture CMV DNA assay (HCA) was evaluated in monitoring a group of renal transplant patients for six months follow up. In this study, the sensitivity, specificity, positive predictive value, and negative predictive value of nested CMV DNA PCR assay and HCA at the ...

Immunosuppressive status in solid organ transplant recipients is often related to the reactivation of Human Cytomegalovirus (HCMV) infection that remains one of the major causes of morbidity and mortality. Therefore, the early detection of HCMV followed by infection monitoring is important to institute prompt and appropriate treatment. In recent years good results have been obtained by HCMV DNA...

background: nematodes are among the most common and important parasites of man and animal. dna of a single worm can be used for several purposes, such as identification to the species, subspecies, strain and antihelmenthic resistance. dna extraction from a single small worm using traditional methods such as phenol extraction technique faces serious prob lems. methods: dna from 20 single haemonc...