background : neospora caninum is considered one of the major causes of repeated abortions in livestock. this study aimed to determine the seropositivity to n. cani-num using indirect elisa and the influence of the infection on the occurrence of abortions in selected dairy herd in slovakia. method: blood samples were obtained from 490 cattle over a period of two years and were tested for n. cani...

BACKGROUND Equine besnoitiosis, caused by Besnoitia bennetti, and equine protozoal myeloencephalitis (EPM), caused by Sarcocystis neurona and Neospora hughesi are relevant equine diseases in the Americas that have been scarcely studied in Europe. Thus, a serosurvey of these cystogenic coccidia was carried out in Southern Spain. A cross-sectional study was performed and serum samples from horses...

Neospora caninum, a protozoan parasite closely related to Toxoplasma gondii, causes abortion and congenital infection in cattle. To investigate specific methods of antemortem diagnosis, the antibody responses of infected cows were evaluated by immunoblot assay and competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) by using a monoclonal antibody (MAb), MAb 4A4-2, against N. cani...

Neospora caninum and Toxoplasma gondii are closely related intracellular protozoan parasites associated with bovine and ovine abortion respectively. Little is known about the extent of Neospora/Toxoplasma co-infection in naturally infected populations of animals. Using nested PCR techniques, based on primers from the Nc5 region of N. caninum and SAG1 for T. gondii, the prevalence of N. caninum ...

Abstract Between December 2016 and April 2017, a spate of abortions occurred in closed dairy herd from the central eastern region Paraná, Brazil, which 75 cows aborted. To identify its cause, organ fragments were collected an aborted fetus for histopathology, blood samples stillborn, 4 fetuses, 9 farm dogs indirect fluorescent antibody technique (IFAT). These tests found multifocal non-suppurat...

background: neospora caninum is an apicomplexan protozoa,which causes abortion in cattle worldwide. objectives:the present study was designed to estimate the rate oftransplacental transmission of n.caninum to aborted fetuses inseropositive dams in dairy farms in mashhad area, iran.methods: two hundred blood samples of dam with abortedbovine fetuses were collected from dairy farms from 2008 to20...

Since its first description in dogs in 1984, the protozoan parasite Neospora caninum has been found to infect a wide range of animals, worldwide. In cattle, N. caninum has particular significance as a cause of abortion in which persistence of infection in the mother, recrudescence of the parasite during pregnancy, and the vulnerability of the placenta and foetus to invasion are important featur...

neospora caninum (n. caninum) is an apicomplexan parasite and causes abortion and congenital neosporosis in cattle worldwide. in this study, we evaluate the virulence of a n. caninum isolate on mouse strain c57bl/6. six groups of five mice c57bl/6 were intraperitoneally inoculated with 1 × l07, 1.5 × l07, 2 × l07, 3 × l07 and 4 × l07 tachyzoites and a control inoculum of dmem, respectively. cli...

Capybara (Hydrochaeris hydrochaeris) is a large rodent distributed throughout tropical America. Antibodies to Neospora caninum in 213 feral capybaras from 11 counties of the State of São Paulo, Brazil, were assessed using the indirect immunofluorescent antibody test (titer >or=1:25) and found in 20 (9.4%), with titers of 1:25 in 4, 1:50 in 7, and 1:100 in 9 animals. This is the first report of ...