The secondary structure and thermal stability of the extrinsic 23 kDa protein (OEC23) of spinach photosystem II have been characterized in solution between 25 and 75 degrees C using Fourier transform infrared spectroscopy. Quantitative analysis of the amide I band (1700-1600 cm(-1)) shows that OEC23 contains 5% alpha-helix, 37% beta-sheet, 24% turn, and 34% disorder structures at 25 degrees C. ...

Visible/near-infraredlaser-induced difference spectroscopy basedon a time-domain terahertz system has beendeveloped, and used to study copperpathancyonine. We find that the absorptionpeak of this molecule at 1.08 THz changessignificantly under 790 nm laserexcitation, suggesting that we haveobserved the first evidence of vibrationalmode changes in the THz range induced byvisible/near-infrared li...

Fourier Transform Infrared (FTIR) spectroscopy is a fundamental technique capable to characterize proteins and to investigate their conformation and dynamics in real physiological environments. Actually, a FTIR spectrum is characterized by many features, which may be correlated to the different components of the protein structure. In the last decade many relevant results have been achieved with...

Infrared spectroscopy is one of the oldest and well established experimental techniques for the analysis of secondary structure of polypeptides and proteins. It is convenient, non-destructive, requires less sample preparation, and can be used under a wide variety of conditions. This review introduces the recent developments in Fourier transform infrared (FTIR) spectroscopy technique and its app...

The evolution of papermaking from XII century, in Europe, was characterized by continuous changes of fibrous and non-fibrous materials as cellulose, wood pulp, sizing agents, fillers and coatings. In this study, the composition of many ancient paper documents is analysed by means of non-destructive Fourier Transform Infrared Spectroscopy (FTIR) in order to identify the main components of paper ...

This paper deals with the problem of tracking an object from a sequence of images captured from a camera moving towards the object. Many conventional image based trackers trace key reference points over an image sequence using simple correlation processes 1]. However, the presence of noise and the magniication of image features as the camera approaches the object may cause a tracked point to dr...

0. Abstract In this paper we propose a simple enhancing protocol for pulsed infrared thermography. The method is based on stimulating the specimen with two heating pulses separated by a short time interval delta. This approach was tested on several specimens of low and high thermal diffusivity and showed to provide higher thermal contrasts when an optimum interval delta is chosen. The observed ...

A new electrochemical model has been carefully established to explain the carbonation behavior of cement mortar, and the model has been validated by the experimental results. In fact, it is shown by this study that the electrochemical impedance behavior of mortars varies in the process of carbonation. With the cement/sand ratio reduced, the carbonation rate reveals more remarkable. The carbonat...

BIRD's eye view: Adding periodic BIRD J-refocusing (BIRD=bilinear rotation decoupling) to data acquisition in an HSQC experiment causes broadband homonuclear decoupling, giving a single signal for each proton chemical shift. This pure shift method improves both resolution and signal-to-noise ratio, without the need for special data processing.