Natural killer (NK) cell number and cytotoxicity are decreased in the blood of patients with a wide range auto-immune conditions. However, role NK cells dermatomyositis (DM) has not been explored. To quantify activity, we utilized signature score, summing standardized z-scores previously validated NK-specific gene set: Nkp46, CD160, KLRC3, CD244, KLRF1, KLRC1, GNLY, PRF1, XCL2, IL18RAP. Upregul...

BACKGROUND Browne et al. [Browne, MacCallum, Kim, Andersen, Glaser: When fit indices and residuals are incompatible. Psychol Methods 2002] employed a structural equation model of measurements of target cell lysing by natural killer cells as an example purportedly demonstrating that small but statistically significant ill model fit can be dismissed as "negligible from a practical point of view"....

The killing activity of natural killer cells is largely regulated by the binding of class I human leukocyte antigen cognate ligands to killer cell immunoglobulin - like receptor proteins. The killer cell immunoglobulin - like receptor gene - complex contains genes that activate and others that inhibit the killing state of natural killer cells depending on the binding of specific human leukocyte...

Background: Perforin is known to be important in cytolytic activity mediated by natural killer (NK) cells.   Objective: To study the relationship between the efficiency of NK and lymphokine-activated killer (LAK) cells activity, and the expression of perforin and HLA class I molecules.   Methods: LAK cells were generated by in vitro culturing of human peripheral blood lymphocytes (PBLs) in the ...

background: perforin is known to be important in cytolytic activity mediated by natural killer (nk) cells.   objective: to study the relationship between the efficiency of nk and lymphokine-activated killer (lak) cells activity, and the expression of perforin and hla class i molecules.   methods: lak cells were generated by in vitro culturing of human peripheral blood lymphocytes (pbls) in the ...

NK assay using flow cytometry. J Immunol Methods 1986; 86: 7-15. 7 Abo T, Balch C M. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol 1981; 127: 1024-9. 8 Lanier L L, Le A M, Phillips J H, Warner N L, Babcock G F. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol ...

Peripheral blood leukemia cells from patients with acute monoblastic leukemia (AMoL) were tested for killer cell activity against target cells that detected natural killer cell-mediated or monocyte-mediated spontaneous cytotoxicity. The fibrosarcoma cell line Wehi 164, pretreated with actinomycin D to induce susceptibility to lysis, specifically detects the activity of unstimulated human monocy...