Chemical modification of proteins has been and continues to be an important biochemical tool for the study of protein structure and function. One such type of approach has been the reductive methylation of lysine residues. In order to address the consequences of such methylation on the crystallization and structural properties of a protein, the three-dimensional structure of hen egg white lysoz...

objective: the experiment was conducted to evaluate the effect of different levels of germinated barley on performance and egg quality in laying hen. methods: one hundred and twenty laying hens, 44-weeks old, lohmann lsl hens were used in this experiment. birds were assigned randomly to the groups, each of which consisted of 20 birds (4 replicate cages each containing 5 birds). the experiment w...

In the past, the toxicity of bacterial lipopolysaccharide (LPS) or its principal bioactive component, lipid A, has detracted from their potential use as radioprotectants. Recently, a relatively nontoxic monophosphoryl Lipid A (LAM) that retains many of the immunobiologic properties of LPS has been isolated from a polysaccharide deficient Re mutant strain of Salmonella minnesota (R595). The abil...

Natural abundance 13C Fourier transform NMR spectra (at 15.18 MHz, in 20-mm sample tubes) of aqueous native proteins yield numerous narrow single carbon resonances of nonprotonated aromatic carbons. Techniques for the assignment of these resonances are presented. Each technique is applied to one or more of the following proteins: ferricytochrome c from horse heart and Candida krusei, ferrocytoc...

We investigated the robustness of hen lysozyme by using random mutant libraries. Six random mutant libraries containing 1, 1.5, 2, 3, 5 and 14 amino acid mutations per hen lysozyme were systematically constructed by varying the concentrations of Mg(2+) and Mn(2+) on polymerase chain reaction. The mutated genes from the six libraries were cloned to a yeast expression vector and a total of 4000 c...

In this study, various molecular dynamics simulations were conducted to investigate the effect of supercritical carbon dioxide on the structural integrity of hen egg white lysozyme. The analyses of backbone root-mean-square deviation, radius of gyration, and secondary structure stability all show that supercritical CO(2) exhibits the ability to increase the stability of this protein, probably a...

Basic physiology, hematology and serum chemistry of indigenous chicken, reared in eco-friendly environment of backyard of small farmers in temperate regions of J and K State (altitude range 1500-2000 m amsl, average temperature 13°C), was studied in adult chicken from different pockets of Kashmir. The average values for haemoglobin (Hb), Packed Cell Volume (PCV), Total Leukocyte Count (TLC), To...

Different types (light to heavy) of laying hens are used in practice. There are questions about the optimum level of balanced protein (BP) supply in feed for different types of hens. Therefore, a broad range of amino acids intake levels [550 to 800 mg of true fecal digestible (TFD) Lys/hen per d] was tested on heavy (Lohmann Brown Classic) and light (Lohmann LSL Classic) laying hens from 24 to ...

The successful resolution of human-wildlife conflicts requires the participation of local communities and other stakeholder groups in formulating management decisions. In the uplands of the United Kingdom, a controversial conservation issue concerns the relationship between the conservation of a legally protected raptor, the Hen Harrier (Circus cyaneus) and the management of a gamebird, the Red...

The oxidative refolding of hen lysozyme has been studied by a variety of time-resolved biophysical methods in conjunction with analysis of folding intermediates using reverse-phase HPLC. In order to achieve this, refolding conditions were designed to reduce aggregation during the early stages of the folding reaction. A complex ensemble of relatively unstructured intermediates with on average tw...