We have investigated, with osmotic techniques, the light-regulated Na+ channels in rod outer segments (ROS) and ROS fragments freshly isolated from the frog retina. Values of Na+ permeability (PNa) similar to those observed electrophysiologically in the retina were observed using the osmotic technique (continuous flow) described by Korenbrot and Cone. In the other osmotic techniques that we exp...

In Ca-deficient smooth muscle of guinea-pig taenia coli, repeated application of high-K solution (45.4 mM) containing Ca induced contractions of similar shape and magnitude. In muscle treated by a Ca- and Mg-deficient solution, however, addition of Ca and K either did not induce contraction or induced only a delayed contraction. Ouabain (1 X 10(-3) M) also inhibited Ca- and K-induced contractio...

In the present study, crystal structures of non-chelating EDTA molecules and their non-chelation salts in a zwitterionic state, along with EDTA-chelates alkali alkaline earth metals, were searched overviewed. 25 EDTA, zwitterions ethylenediammonium-diacetate diacetic acid HOOC-CH2-(-OOC-CH2-)NH+-CH2-CH2-NH+(-CH2-COO-)-CH2-COOH (ethylenediammonium-tetraace­tic (HOOC-CH2-)2NH+-CH2-CH2-NH+(-CH2-CO...

EDTA can complex with radionuclides (RNs) to form negatively charged complexes, making it difficult for clay minerals retard the diffusion of RNs waste. The coefficient in water ( D w ) is an important parameter safety assessment repository. In this study, effectsof on metal ions (Cu 2+ , Sm 3+ Nd Lu and Zn were investigated by a capillary method. experimental results showed that [Cu-EDTA] 2- [...

A series of batch tests were conducted to investigate the relative desorption potential of mercury from kaolin and glacial till soils under the effect of three extractant agents, namely, KI, NaEDTA and NaCl (all of them with a concentration of 0.1 M). These tests were conducted under different pH conditions (within a pH range between 2 and 12). Also batch tests using deionized water were perfor...

When sperm of Strongylocentrotus purpuratus or Lytechinus pictus are diluted into seawater, motility is initiated; and when exposed to egg jelly, an acrosome reaction is induced. In the presence of a variety of structurally different metal chelators (0.1-1 mM EDTA, EGTA, phenanthroline, dipyridyl, cysteine, or dithiothreitol), motility initiation is delayed and the acrosome reaction is inhibite...

Induction of outer membrane protein H1 in Pseudomonas aeruginosa results in decreased susceptibility to aminoglycosides, polymyxin B, and EDTA. We have previously shown that protein H1 can become the major cellular protein in cells grown in low (0.02 mM) Mg2+. The induction of protein H1 was prevented by supplementation of low Mg2+ medium with Mg2+, Ca2+, Mn2+, or Sr2+ (each at 0.5 mM), but not...

in this study, the chemical factors effect of metal ions, surfactant, oxidizing agents and enzyme inhibitors on the activity of trypsin and chymotrypsin of rainbow trout fry were considered. the results showed that k+ and na+ had not significant decrease on trypsin and chymotrypsin activity than did those from control (p>0.05). ca2+ and mg2+ showed a significant increase in activity of trypsin ...