The design of fully or partly defined media for mycoplasma cultivation involves the need to provide the essential lipids, cholesterol and long-chain fatty acids, in an assimilable and nontoxic form. This study introduces cyclodextrins (CDs) as carriers of these lipids, thus suggesting alternatives to serum or bovine serum albumin (BSA). The effects of beta-CD and two forms of chemically modifie...

mastitis, arthritis, keratoconjunctivitis and, occasionally, abortion. Mycoplasma agalactiae (Ma) is the main cause of the disease in sheep and goats, but M. capricolum subsp. capricolum (Mcc), M. mycoides subsp. capri (Mmc) and M. putrefaciens produce a clinically similar disease, more often in goats, which may be accompanied by pneumonia. Ma and Mcc have been isolated from wild small ruminant...

The detection of mycoplasma in milk can be performed by either culture techniques or polymerase chain reaction (PCR) based methods. Although PCR can reduce the average diagnostic time to 5 h in comparison with the several days for the isolation of the agent, there is still a need to develop methods, which could give earlier results. For this purpose, we tested the ability of flow cytometry (FC)...

  I n a survey of pneumonia due to Mycoplasma 282 out of 12168 ovine and caprine lung condemnation were collected (2.32%). Mycoplasma spp. has been isolated from pneumonic cases in 4 sheep and 2 goats. PCR studies were confirmed the genus of Mycoplasma although attempting for identification of strains M. mycoides, M. capricolum/caprine pleuropneumonia and M. arginin were in failure. The lesions...

1. Nicholas R, Churchward C. Contagious caprine pleuropneumonia: new aspects of an old disease. Transbound Emerg Dis. 2012;59:189–96. http://dx.doi. org/10.1111/j.1865-1682.2011.01262.x 2. Fischer A, Shapiro B, Muriuki C, Heller M, Schnee C, Bongcam-Rudloff E, et al. The origin of the ‘Mycoplasma mycoides cluster’ coincides with domestication of ruminants. PLoS ONE. 2012;7:e36150. h t tp : / /d...

BACKGROUND The three-dimensional structures of histidine-containing phosphocarrier protein (HPr), a member of the phosphoenolpyruvate:sugar phosphotransferase system (PTS), have been determined from Gram-negative and Gram-positive bacteria. The structure of HPr reported here for Mycoplasma capricolum is the first protein structure to be determined for this class of organism. Comparative structu...