Two new Ca indicators, purpurate-3,3'diacetic acid (PDAA) and 1,1'-dimethylpurpurate-3,3'diacetic acid (DMPDAA), were synthesized and used to measure Ca transients in frog cut muscle fibers. These indicators are analogues of the purpurate components of murexide and tetramethylmurexide, in which two acetate groups have been incorporated into each molecule to render it membrane impermeant. The ap...

The activation of factor VIII:C by thrombin appears to be an important prerequisite for the function of factor VIII:C as a cofactor in factor X activation in coagulation. The possible modulation of factor VIII:C activation by potential cofactors such as calcium ions, phospholipid, and platelets was studied systematically. Factor VIII:C activation could not be studied in the complete absence of ...

Visceral urate deposition (visceral gout) is a common finding during post-mortem examination of poultry. Rare cases of visceral gout may occur in wild birds. A rare case of visceral urate deposition in a little bittern (Ixobrychus minutus) is reported here. In May 2013, carcass of a little bittern was submitted for necropsy to the Clinic of Poultry Diseases (Faculty of Veterinary Medic...

At the concentrations usually employed as a Ca2+ indicator, arsenazo III undergoes a one-electron reduction by rat liver microsomes to produce an azo anion radical as demonstrated by electron spin resonance spectroscopy. Either NADH or NADPH can serve as a source of reducing equivalents for the production of this free radical by rat liver microsomes. The steady state concentration of the azo an...

The sequence of contraction-relaxation for myocardial cells is believed to be linked to Ca2+ flux across the sarcoplasmic reticulum. Alterations of sarcoplasmic reticulum function during ischemia may result in depressing the myocardial contraction-relaxation sequence. This study examines the relationship betw een the m em brane potential and Ca2+ flux across sar­ coplasm ic reticu lum vesicles ...

visceral urate deposition (visceral gout) is a common finding during post-mortem examination of poultry. rare cases of visceral gout may occur in wild birds. a rare case of visceral urate deposition in a little bittern (ixobrychus minutus) is reported here. in may 2013, carcass of a little bittern was submitted for necropsy to the clinic of poultry diseases (faculty of veterinary medicine, razi...

CHE~IICAL METHOD. The estimation of cafeine. Older methods for the determination of car7eine in biological material involve extraction into chloroform, followed by assay of the drug by its nitrogen content (Kunz, 1935) or by a color-producing murexide reaction (Tanaka and Ohkubo, 1937). These methods are insensitive and inaccurate. Recently, Ishler et al. (1948) described a sensitive method, us...

Ca 2+, Mg2+-ionophores X537A and A23,187 (10-r-10 -6 M) induced the release of adenine nucleotides adenosine diphosphate (ADP, adenosine triphosphate (ATP), serotonin, /3-glucuronidase, Ca 2+, and Mg 2+ from washed human platelets. Enzymes present in the cytoplasm or mitochondria, and Zn 2+ were not released. The rate of ATP and Ca 2+ release measured by firefly lantern extract and murexide dye...

Groundwater in Bangladesh is contaminated with Arsenic, which occurs naturally in alluvial and deltaic sediments. The first official detection in 1993 and subsequent confirmation after 1995 of high levels of Arsenic in numerous shallow and deep wells in various parts of the country has raised serious health concerns. Recent investigations, though incomplete, confirm that the occurrence of Arsen...