A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ, fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonel...

Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining various combinations of those primer sets and different reaction components and/or thermal cycling conditions. The process of optimizing a mul...

Multiplex networks are a type of multilayer network in which entities are connected to each other via multiple types of connections. We propose a method, based on computing pairwise similarities between layers and then doing community detection, for grouping structurally similar layers in multiplex networks. We illustrate our approach using both synthetic and empirical networks, and we are able...

Here we present a novel microfluidic design to generate multiplex gradients of biomolecules based on hydrodynamic resistances. Chemical gradients were generated within parallel microfluidic channels, in which a range of multiplex concentration gradients with different profile shapes were simultaneously produced. We could also successfully generate covalent surface gradients of biomolecules. IgG...

clostridium perfringens is an important cause of bacterial food poisoning worldwide. the disease is caused by c. perfringens enterotoxin (cpe) encoded by cpe gene. the aim of this research was to identify the different types of c. perfringens and the presence of cpe gene in isolated bacteria from broilers’ meat marketed in retail meat shops of mashhad city in northeastern of iran. after isolati...

conclusions as about 70% of methicillin-resistant isolates belonged to sccmec iii, the present study can conclude that, over an 8-year period, only one dominant and stable clone of mrsa strain was found in tabriz hospitals. this finding could be a result of incorrect medical orientations, inadequate infection controlling policies, and insufficient preventive approaches. objectives the current s...

Multiplex networks describe a large variety of complex systems, whose elements (nodes) can be connected by different types of interactions forming different layers (networks) of the multiplex. Multiplex networks include social networks, transportation networks, or biological networks in the cell or in the brain. Extracting relevant information from these networks is of crucial importance for so...